Change of protein patterns induced by photooxidative stress
Changes in protein patterns of Rhodobacter sphaeroides are utilized by the Microbiology and Molecular Biology Group (Prof. Klug) to investigate regulative responses to photooxidative stress. Proteins are marked in vivo by 35S-Methionin and separated by 2D gel electrophoresis. This way, differences in protein sythesis can be visualized.
Proteins synthesized after singlet oxygen exposure are marked red. and blue spots represent proteins from control treatment. If proteins are synthesized to the same extent under both conditions the staining adds to black.
Jens Glaeser, Monica Zobawa, Friedrich Lottspeich, Gabriele Klug (2007) Protein Synthesis Patterns Reveal a Complex Regulatory Response to Singlet Oxygen in Rhodobacter. J. Proteome Res., 6 (7), 2460 -2471