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Sie sind hier: Startseite Prof. Dr. A. Pingoud Publikationen
Artikelaktionen

Publikationen

Anbei die Publikationen der AG Pingoud der letzten fünf Jahre.

Pingoud

 
Chemical rescue of active site mutants of the S. pneumoniae surface endonuclease EndA and other nucleases of the H-N-H family by imidazole
Marika Midon, Oleg Gimadutdinow, Gregor Meiss, Peter Friedhoff, and Alfred Pingoud
ChemBioChem, in press (doi: 10.1002/cbic.201100775)
 
The rotation-coupled sliding of EcoRV.
Dikic J, Menges C, Clarke S, Kokkinidis M, Pingoud A, Wende W, Desbiolles P.
Nucl. Acids Res. 2012 Jan 12. [Epub ahead of print] doi: 10.1093/nar/gkr1309
Free pdf file

A novel zinc-finger nuclease platform with a sequence-specific cleavage module.
Schierling B, Dannemann N, Gabsalilow L, Wende W, Cathomen T, Pingoud A.
Nucleic Acids Res., in press (doi:10.1093/nar/gkr1112)
 
Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.
Fonfara I, Curth U, Pingoud A, Wende W.
Nucleic Acids Res. 2011 Sep 29. [Epub ahead of print]
PMID: 21965534
Free pdf file
 
Restriction endonuclease SsoII with photoregulated activity - a "molecular gate" approach.
Hien le T, Zatsepin TS, Schierling B, Volkov EM, Wende W, Pingoud A, Kubareva EA, Oretskaya TS.
Bioconjug Chem. 2011 Jul 20;22(7):1366-73. Epub 2011 Jul 1.
PMID: 21688832 [PubMed - in process]
Free pdf file
 
Generation of novel nucleases with extended specificity by rational and combinatorial strategies.
Pingoud A, Wende W.
Chembiochem. 2011 Jul 4;12(10):1495-500. doi: 10.1002/cbic.201100055. Epub 2011 May 10. No abstract available.
PMID: 21560218 [PubMed - in process]
Free pdf file
 
Photocaged Variants of the MunI and PvuII Restriction Enzymes.
Silanskas A, Foss M, Wende W, Urbanke C, Lagunavicius A, Pingoud A, Siksnys V.
Biochemistry. 2011 Apr 12;50(14):2800-7. Epub 2011 Mar 16.
PMID: 21410225 [PubMed - in process] 
Free pdf file

Structural insights into catalytic and substrate binding mechanisms of the strategic EndA nuclease from Streptococcus pneumoniae.
Moon AF, Midon M, Meiss G, Pingoud A, London RE, Pedersen LC.
Nucleic Acids Res. 2011 Apr 1;39(7):2943-53. Epub 2010 Nov 26.
PMID: 21113026 [PubMed - in process] 
Free pdf file

Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae.
Midon M, Schäfer P, Pingoud A, Ghosh M, Moon AF, Cuneo MJ, London RE, Meiss G.
Nucleic Acids Res. 2011 Jan;39(2):623-34. Epub 2010 Sep 15.
PMID: 20846957 [PubMed - indexed for MEDLINE] 
Free pdf file

Controlling the enzymatic activity of a restriction enzyme by light.
Schierling B, Noël AJ, Wende W, Hien le T, Volkov E, Kubareva E, Oretskaya T, Kokkinidis M, Römpp A, Spengler B, Pingoud A.
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1361-6. Epub 2009 Dec 18.
PMID: 20080559 [PubMed - indexed for MEDLINE] 
Free pdf file

[New derivatives of azobenzene for the directed modification of proteins].
Khien le T, Shirling B, Riazanova AIu, Zatsepin TS, Volkov EM, Kubareva EA, Velichko TI, Pingoud A, Oretskaia TS.
Bioorg Khim. 2009 Sep-Oct;35(5):610-7. Russian. 
PMID: 19915638 [PubMed - indexed for MEDLINE] 

On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.
Pingoud V, Wende W, Friedhoff P, Reuter M, Alves J, Jeltsch A, Mones L, Fuxreiter M, Pingoud A.
J Mol Biol. 2009 Oct 16;393(1):140-60. Epub 2009 Aug 13.
PMID: 19682999 [PubMed - indexed for MEDLINE] 
Free pdf file

Characterization of Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease, reveals a unique mode of DNA binding, helical distortion, and cleavage compared with a canonical LAGLIDADG homing endonuclease.
Singh P, Tripathi P, Silva GH, Pingoud A, Muniyappa K.
J Biol Chem. 2009 Sep 18;284(38):25912-28. Epub 2009 Jul 15.
PMID: 19605345 [PubMed - indexed for MEDLINE] 
Free full text 

Endonucleases induced TRAIL-insensitive apoptosis in ovarian carcinoma cells.
Geel TM, Meiss G, van der Gun BT, Kroesen BJ, de Leij LF, Zaremba M, Silanskas A, Kokkinidis M, Pingoud A, Ruiters MH, McLaughlin PM, Rots MG.
Exp Cell Res. 2009 Sep 10;315(15):2487-95. Epub 2009 Jun 18.
PMID: 19540229 [PubMed - indexed for MEDLINE] 
Free pdf file

Tracking of single quantum dot labeled EcoRV sliding along DNA manipulated by double optical tweezers.
Biebricher A, Wende W, Escudé C, Pingoud A, Desbiolles P.
Biophys J. 2009 Apr 22;96(8):L50-2.
PMID: 19383444 [PubMed - indexed for MEDLINE] 
Free pdf file

Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA.
Bonnet I, Biebricher A, Porté PL, Loverdo C, Bénichou O, Voituriez R, Escudé C, Wende W, Pingoud A, Desbiolles P.
Nucleic Acids Res. 2008 Jul;36(12):4118-27. Epub 2008 Jun 10.
PMID: 18544605 [PubMed - indexed for MEDLINE] 
Free pdf file

Precision genome surgery.
Pingoud A, Silva GH.
Nat Biotechnol. 2007 Jul;25(7):743-4. No abstract available. 
PMID: 17621297 [PubMed - indexed for MEDLINE] 
Free pdf file

A sliding restriction enzyme pauses.
Pingoud A, Wende W.
Structure. 2007 Apr;15(4):391-3. No abstract available. 
PMID: 17437711 [PubMed - indexed for MEDLINE] 
Free pdf file

Reversible inactivation of the CG specific SssI DNA (cytosine-C5)-methyltransferase with a photocleavable protecting group.
Rathert P, Raskó T, Roth M, Slaska-Kiss K, Pingoud A, Kiss A, Jeltsch A.
Chembiochem. 2007 Jan 22;8(2):202-7.
PMID: 17195251 [PubMed - indexed for MEDLINE] 
Free pdf file

The nuclease a-inhibitor complex is characterized by a novel metal ion bridge.
Ghosh M, Meiss G, Pingoud AM, London RE, Pedersen LC.
J Biol Chem. 2007 Feb 23;282(8):5682-90. Epub 2006 Nov 30.
PMID: 17138564 [PubMed - indexed for MEDLINE] 
Free pdf file

Engineering site-specific endonucleases.
Friedhoff P, Pingoud A.
Methods Mol Biol. 2007;352:111-23.
PMID: 17041262 [PubMed - indexed for MEDLINE] 

Identification of base-specific contacts in protein-DNA complexes by photocrosslinking and mass spectrometry: a case study using the restriction endonuclease SsoII.
Pingoud V, Geyer H, Geyer R, Kubareva E, Bujnicki JM, Pingoud A.
Mol Biosyst. 2005 Jul;1(2):135-41. Epub 2005 Jun 9.
PMID: 16880975 [PubMed - indexed for MEDLINE] 
Free pdf file

From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes.
Silva GH, Belfort M, Wende W, Pingoud A.
J Mol Biol. 2006 Aug 25;361(4):744-54. Epub 2006 Jul 12.
PMID: 16872628 [PubMed - indexed for MEDLINE] 
Free pdf file

Developing a programmed restriction endonuclease for highly specific DNA cleavage.
Eisenschmidt K, Lanio T, Simoncsits A, Jeltsch A, Pingoud V, Wende W, Pingoud A.
Nucleic Acids Res. 2005 Dec 14;33(22):7039-47. Print 2005.
PMID: 16356926 [PubMed - indexed for MEDLINE] 
Free pdf file

Affinity modification of the restriction endonuclease SsoII by 2'-aldehyde-containing double stranded DNAs.
Sud'ina AE, Zatsepin TS, Pingoud V, Pingoud A, Oretskaya TS, Kubareva EA.
Biochemistry (Mosc). 2005 Aug;70(8):941-7.
PMID: 16212552 [PubMed - indexed for MEDLINE] 
Free pdf file

Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena.
Ghosh M, Meiss G, Pingoud A, London RE, Pedersen LC.
J Biol Chem. 2005 Jul 29;280(30):27990-7. Epub 2005 May 15.
PMID: 15897201 [PubMed - indexed for MEDLINE] 
Free pdf file

Type II restriction endonucleases: structure and mechanism.
Pingoud A, Fuxreiter M, Pingoud V, Wende W.
Cell Mol Life Sci. 2005 Mar;62(6):685-707. Review.
PMID: 15770420 [PubMed - indexed for MEDLINE] 
Free pdf file

Interaction of DNA fragmentation factor (DFF) with DNA reveals an unprecedented mechanism for nuclease inhibition and suggests that DFF can be activated in a DNA-bound state.
Korn C, Scholz SR, Gimadutdinow O, Lurz R, Pingoud A, Meiss G.
J Biol Chem. 2005 Feb 18;280(7):6005-15. Epub 2004 Nov 30.
PMID: 15572351 [PubMed - indexed for MEDLINE] 
Free pdf file

Specificity changes in the evolution of type II restriction endonucleases: a biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences.
Pingoud V, Sudina A, Geyer H, Bujnicki JM, Lurz R, Lüder G, Morgan R, Kubareva E, Pingoud A.
J Biol Chem. 2005 Feb 11;280(6):4289-98. Epub 2004 Nov 24.
PMID: 15563460 [PubMed - indexed for MEDLINE] 
Free pdf file

Friedhoff

 
Chemical rescue of active site mutants of the S. pneumoniae surface endonuclease EndA and other nucleases of the H-N-H family by imidazole
Marika Midon, Oleg Gimadutdinow, Gregor Meiss, Peter Friedhoff, and Alfred Pingoud
ChemBioChem, in press (doi: 10.1002/cbic.201100775)
 
Chemical trapping of the dynamic MUTS-MUTL complex formed in DNA mismatch repair in Escherichia coli.
Winkler I, Marx AD, Lariviere D, Heinze R, Cristovao M, Reumer A, Curth U, Sixma TK, Friedhoff P.
J Biol Chem. 2011 Mar 15. [Epub ahead of print]
PMID: 21454657 [PubMed - as supplied by publisher] 
Free pdf file

Changes in cytosolic Mg2+ levels can regulate the activity of the plasma membrane H+-ATPase in maize.
Hanstein S, Wang X, Qian X, Friedhoff P, Fatima A, Shan Y, Feng K, Schubert S.
Biochem J. 2011 Apr 1;435(1):93-101.
PMID: 21247408 [PubMed - in process] 
Free pdf file

[Secondary structure of SsoII-like (cytosine-5)-DNA methyltransferases N-terminal region determined by circular dichroism spectroscopy].
Riazanova AIu, Molochkov NV, Abrosimova LA, Alekseevskiĭ AV, Kariagina AS, Protsenko AS, Friedhoff P, Oretskaia TS, Kubareva EA.
Mol Biol (Mosk). 2010 Sep-Oct;44(5):911-21. Russian. 
PMID: 21090246 [PubMed - indexed for MEDLINE] 

The C-terminal domain of the MutL homolog from Neisseria gonorrhoeae forms an inverted homodimer.
Namadurai S, Jain D, Kulkarni DS, Tabib CR, Friedhoff P, Rao DN, Nair DT.
PLoS One. 2010 Oct 28;5(10):e13726.
PMID: 21060849 [PubMed - indexed for MEDLINE] 
Free pdf file

Structure of the endonuclease domain of MutL: unlicensed to cut.
Pillon MC, Lorenowicz JJ, Uckelmann M, Klocko AD, Mitchell RR, Chung YS, Modrich P, Walker GC, Simmons LA, Friedhoff P, Guarné A.
Mol Cell. 2010 Jul 9;39(1):145-51.
PMID: 20603082 [PubMed - indexed for MEDLINE] 
Free pdf file

Identification of Lynch syndrome mutations in the MLH1-PMS2 interface that disturb dimerization and mismatch repair.
Kosinski J, Hinrichsen I, Bujnicki JM, Friedhoff P, Plotz G.
Hum Mutat. 2010 Aug;31(8):975-82.
PMID: 20533529 [PubMed - indexed for MEDLINE] 
Free pdf file

Maintaining a sense of direction during long-range communication on DNA.
Szczelkun MD, Friedhoff P, Seidel R.
Biochem Soc Trans. 2010 Apr;38(2):404-9. Review.
PMID: 20298192 [PubMed - indexed for MEDLINE] 
Free pdf file

On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.
Pingoud V, Wende W, Friedhoff P, Reuter M, Alves J, Jeltsch A, Mones L, Fuxreiter M, Pingoud A.
J Mol Biol. 2009 Oct 16;393(1):140-60. Epub 2009 Aug 13.
PMID: 19682999 [PubMed - indexed for MEDLINE] 
Free pdf file

The C-terminal domain is sufficient for endonuclease activity of Neisseria gonorrhoeae MutL.
Duppatla V, Bodda C, Urbanke C, Friedhoff P, Rao DN.
Biochem J. 2009 Sep 25;423(2):265-77.
PMID: 19656086 [PubMed - indexed for MEDLINE] 
Free pdf file

Physical and functional interactions between Escherichia coli MutL and the Vsr repair endonuclease.
Heinze RJ, Giron-Monzon L, Solovyova A, Elliot SL, Geisler S, Cupples CG, Connolly BA, Friedhoff P.
Nucleic Acids Res. 2009 Jul;37(13):4453-63. Epub 2009 May 27.
PMID: 19474347 [PubMed - indexed for MEDLINE] 
Free pdf file

The PMS2 subunit of human MutLalpha contains a metal ion binding domain of the iron-dependent repressor protein family.
Kosinski J, Plotz G, Guarné A, Bujnicki JM, Friedhoff P.
J Mol Biol. 2008 Oct 10;382(3):610-27. Epub 2008 Jun 28.
PMID: 18619468 [PubMed - indexed for MEDLINE] 
Free pdf file

Mutations in the MutSalpha interaction interface of MLH1 can abolish DNA mismatch repair.
Plotz G, Welsch C, Giron-Monzon L, Friedhoff P, Albrecht M, Piiper A, Biondi RM, Lengauer T, Zeuzem S, Raedle J.
Nucleic Acids Res. 2006;34(22):6574-86. Epub 2006 Nov 28.
PMID: 17135187 [PubMed - indexed for MEDLINE] 
Free pdf file

Engineering site-specific endonucleases.
Friedhoff P, Pingoud A.
Methods Mol Biol. 2007;352:111-23.
PMID: 17041262 [PubMed - indexed for MEDLINE] 

Structural and functional analysis of the MutS C-terminal tetramerization domain.
Manelyte L, Urbanke C, Giron-Monzon L, Friedhoff P.
Nucleic Acids Res. 2006;34(18):5270-9. Epub 2006 Sep 29.
PMID: 17012287 [PubMed - indexed for MEDLINE] 
Free pdf file
 
Identifying an interaction site between MutH and the C-terminal domain of MutL by crosslinking, affinity purification, chemical coding and mass spectrometry.
Ahrends R, Kosinski J, Kirsch D, Manelyte L, Giron-Monzon L, Hummerich L, Schulz O, Spengler B, Friedhoff P.
Nucleic Acids Res. 2006 Jun 13;34(10):3169-80. Print 2006.
PMID: 16772401 [PubMed - indexed for MEDLINE] 
Free pdf file

Analysis of the quaternary structure of the MutL C-terminal domain.
Kosinski J, Steindorf I, Bujnicki JM, Giron-Monzon L, Friedhoff P.
J Mol Biol. 2005 Aug 26;351(4):895-909.
PMID: 16024043 [PubMed - indexed for MEDLINE] 
Free pdf file
 
Mapping protein-protein interactions by bioinformatics and cross-linking.
Friedhoff P.
Anal Bioanal Chem. 2005 Jan;381(1):78-80. No abstract available. 
PMID: 15614498 [PubMed - indexed for MEDLINE] 
Free pdf file
 

Wende

 
The rotation-coupled sliding of EcoRV.
Dikic J, Menges C, Clarke S, Kokkinidis M, Pingoud A, Wende W, Desbiolles P.
Nucl. Acids Res. 2012 Jan 12. [Epub ahead of print] doi: 10.1093/nar/gkr1309
Free pdf file
 
A novel zinc-finger nuclease platform with a sequence-specific cleavage module.
Schierling B, Dannemann N, Gabsalilow L, Wende W, Cathomen T, Pingoud A.
Nucleic Acids Res. in press (doi:10.1093/nar/gkr1112)
 
Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.
Fonfara I, Curth U, Pingoud A, Wende W.
Nucleic Acids Res. 2011 Sep 29. [Epub ahead of print]
PMID: 21965534
Free pdf file
 
Restriction endonuclease SsoII with photoregulated activity - a "molecular gate" approach.
Hien le T, Zatsepin TS, Schierling B, Volkov EM, Wende W, Pingoud A, Kubareva EA, Oretskaya TS.
Bioconjug Chem. 2011 Jul 20;22(7):1366-73. Epub 2011 Jul 1.
PMID: 21688832 [PubMed - in process]
Free pdf file
 
Generation of novel nucleases with extended specificity by rational and combinatorial strategies.
Pingoud A, Wende W.
Chembiochem. 2011 Jul 4;12(10):1495-500. doi: 10.1002/cbic.201100055. Epub 2011 May 10. No abstract available.
PMID: 21560218 [PubMed - in process]
Free pdf file
 
Photocaged Variants of the MunI and PvuII Restriction Enzymes.
Silanskas A, Foss M, Wende W, Urbanke C, Lagunavicius A, Pingoud A, Siksnys V.
Biochemistry. 2011 Apr 12;50(14):2800-7. Epub 2011 Mar 16.
PMID: 21410225 [PubMed - in process] 
Free pdf file

Identification of conserved features of LAGLIDADG homing endonucleases.
Grishin A, Fonfara I, Alexeevski A, Spirin S, Zanegina O, Karyagina A, Alexeyevsky D, Wende W.
J Bioinform Comput Biol. 2010 Jun;8(3):453-69.
PMID: 20556856 [PubMed - indexed for MEDLINE] 

Controlling the enzymatic activity of a restriction enzyme by light.
Schierling B, Noël AJ, Wende W, Hien le T, Volkov E, Kubareva E, Oretskaya T, Kokkinidis M, Römpp A, Spengler B, Pingoud A.
Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1361-6. Epub 2009 Dec 18.
PMID: 20080559 [PubMed - indexed for MEDLINE] 
Free pdf file

On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family.
Pingoud V, Wende W, Friedhoff P, Reuter M, Alves J, Jeltsch A, Mones L, Fuxreiter M, Pingoud A.
J Mol Biol. 2009 Oct 16;393(1):140-60. Epub 2009 Aug 13.
PMID: 19682999 [PubMed - indexed for MEDLINE] 
Free pdf file

Tracking of single quantum dot labeled EcoRV sliding along DNA manipulated by double optical tweezers.
Biebricher A, Wende W, Escudé C, Pingoud A, Desbiolles P.
Biophys J. 2009 Apr 22;96(8):L50-2.
PMID: 19383444 [PubMed - indexed for MEDLINE] 
Free pdf file

Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA.
Bonnet I, Biebricher A, Porté PL, Loverdo C, Bénichou O, Voituriez R, Escudé C, Wende W, Pingoud A, Desbiolles P.
Nucleic Acids Res. 2008 Jul;36(12):4118-27. Epub 2008 Jun 10.
PMID: 18544605 [PubMed - indexed for MEDLINE] 
Free pdf file

A sliding restriction enzyme pauses.
Pingoud A, Wende W.
Structure. 2007 Apr;15(4):391-3. No abstract available. 
PMID: 17437711 [PubMed - indexed for MEDLINE] 
Free pdf file

From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes.
Silva GH, Belfort M, Wende W, Pingoud A.
J Mol Biol. 2006 Aug 25;361(4):744-54. Epub 2006 Jul 12.
PMID: 16872628 [PubMed - indexed for MEDLINE] 
Free pdf file

Plasmodium falciparum glutathione S-transferase--structural and mechanistic studies on ligand binding and enzyme inhibition.
Hiller N, Fritz-Wolf K, Deponte M, Wende W, Zimmermann H, Becker K.
Protein Sci. 2006 Feb;15(2):281-9. Epub 2005 Dec 29.
PMID: 16385005 [PubMed - indexed for MEDLINE] 
Free pdf file

Developing a programmed restriction endonuclease for highly specific DNA cleavage.
Eisenschmidt K, Lanio T, Simoncsits A, Jeltsch A, Pingoud V, Wende W, Pingoud A.
Nucleic Acids Res. 2005 Dec 14;33(22):7039-47. Print 2005.
PMID: 16356926 [PubMed - indexed for MEDLINE] 
Free pdf file

Type II restriction endonucleases: structure and mechanism.
Pingoud A, Fuxreiter M, Pingoud V, Wende W.
Cell Mol Life Sci. 2005 Mar;62(6):685-707. Review.
PMID: 15770420 [PubMed - indexed for MEDLINE] 
Free pdf file


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