Regulation of chromatin structure during meiotic and postmeiotic mammalian germ cell development.
During male germ cell development, pre-meiotic chromosomes recombine, and postmeiotic chromosomes are reorganized to form the compact sperm nucleus. The X and Y chromosomes are subject to special regulation. We want to understand how the ubiquitin-conjugating enzyme HR6B regulates meiotic and postmeiotic chromatin reorganization, and to study the possible role of CTCF. We will analyse meiotic recombination, chromosome pairing, and postmeiotic chromatin structure in testis from transgenic mouse models, and in early embryos through in vitro fertilization techniques.