Workshop on Single Cell Analysis

14:30 - 14:40

Martin Blaser, International Giessen Graduate Centre for Life Sciences (GGL)

GGL Postdoc Program

14:40 - 15:20

Alexander Misharin,  Nortwestern University, Division of Pulmonary and Critical Care

Single cell RNA-Seq analysis of healthy and diseased human lungs

15:20 - 16:00

Andrius Serva, Fluidigm Corporation

Discovery and Functional Profiling with Mass Cytometry - High order multiplexing of biological samples - from suspension to tissue

16:00 - 16:30

Coffee Break

16:30 - 17:10

Martijn C. Nawijn, University of Groningen, Department of Pathology and Medical Biology

Human Cell Atlas Lung Project

17:10 - 17:50

Christos Samakovlis, Justus-Liebig-University Giessen, Department of Internal Medicine

In situ single cell RNA sequencing of lung tissue

09:00 – 09:45

Alexander Misharin,  Nortwestern University, Division of Pulmonary and Critical Care

Basic Principles of FACS Analyses and Cell Sorting

9:45 – 17:00             Hands-on workshop limited - please register

Roxana Wasnick, Justus Liebig University Giessen, Department of Internal Medicine

Intro – basic experimental design for scNGS

Janine Koepke, Justus Liebig University Giessen, Department of Internal Medicine

Cell isolation for scNGS

Ivonne Vasquez, Justus Liebig University Giessen, Department of Internal Medicine, FACS Facility

FACS sorting for single cell experiments

Stefan Günther, MPI for Heart and Lung Disease, Bioinformatics and deep sequencing platform

Cell capture and library preparation and scNGS and basics of data analysis