Research Wende
Wende
Highly specific tailor-made nucleases as essential tools for genome engineering
TALE nucleases and the recently introduced CRISPR-Cas9 system have widely spread and become the methods of choice for genome editing in a wide range of higher organisms. However, critical mechanistic details of the technologies remain largely unknown. Therefore, we investigate the mechanism how these genome targeting tools find their specific target, as well as the thermodynamics and kinetics of DNA binding. Based on these results, we want to improve the specificity of our new designer nucleases and promote their clinical and biotechnological application.
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