Instruments

Our setup is a dual 2-photon system, consisting of an inverted and an upright setup. Both systems can be operated individually as confocal microscopes while they can share a multi-photon laser from SpectraPhysics.

 Inverted microscope

The inverted system has an Acousto-Optical Beam Splitter (AOBS), with a z-galvo stage and a resonant scanner, offering the following:

Confocal Laser Scanning Microscopy

• Lasers:
• 405nm
• Argon laser (458nm, 476nm, 488nm, 496nm and 514nm)
• White Light Laser (470nm-670nm)
• Detectors:
• 1 Transmitted Light PMT
• 1 PMT
• 3 Hybrid Detectors

Multi-photon Laser Scanning Microscopy

• Laser:
• SpectraPhysics Insight X3 with a 1045nm fixed line and a tuned line from 680nm up to 1300nm.
• Detectors:
• 4-Tune Mutli-photon module with 2 PMTs and 2HyDs

Digital Light Sheet

The inverted microscope is also equipped with a light sheet module suitable for small volumes (width up to 5mm).

Objectives

• 1.6x air, 2.5x air, 5x air, 10x air, 20x air
• 25xWater, 40xWater, 63xWater (with motor correction collar)
• 63xOil
• 5x water diving objective for light sheet
• 10x multi-immersion diving objective for light sheet
• 5mm water, 7.8mm water/Glyc/BABB TwinFlect mirrors 

Software Modules

• Lightning for online computational clearing
• FRET and FRAP module
• LiveData Module
• Spectral Dye Separation

Live Imaging Setup

The inverted microscope is also equipped with a temperature controller as well as a gas mixer for CO2, providing the option for live cell imaging.

Upright Microscope

The upright microscope is equipped with a Scientifica XY stage and a resonant scanner for fast imaging and can offer the following:

Confocal Laser Scanning Microscopy

• Lasers:
• 405nm
• 488nm
• 552nm
• 638nm
• Detectors:
• 3 PMT
• 1 Hybrid Detector

Multi-photon Laser Scanning Microscopy

• Laser:
• SpectraPhysics Insight X3 with a 1045nm fixed line and a tuned line from 680nm up to 1300nm.
• Detectors:
• 4-Tune Mutli-photon module with 2 PMTs and 2HyDs

Objectives

• 25x/1.00NA water diving with motor correction collar
• 25x/1.00NA multi-immersion diving with motor correction collar

Software Modules

• Lightning for online computational clearing
• Spectral Dye Separation

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