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MP110 Module Description

Attention: Module MP110 will take place!
It will be either from September 20 to 24, 2021 or (as it currently stands more likely) from September 13 to 17, 2021; you can find more information in StudIP.

Summer semester
Block seminar, usually 3rd week in September, time: 8:30 - 18:00
Room: IFZ B301, MZVG



Special Biochemistry II

2nd or 4th Sem

6 CP


Module name

Special Biochemistry II

Module code   


Faculty / subject / institute

Faculty 09/ Agricultural Sciences, Nutritional Sciences and Environmental Management

Assosiated degree course(s)/semester taken

 Master Agrobiotechnology 1) 2nd or 4th semester

Module coordinator

Prof. Dr. K. Becker


Dr. Stefan Rahlfs and members of the lab




Chemistry I, Biochemistry I, BKE30

Learning outcomes

Students will be able to do the following:

  • Have in-depth knowledge and proficiency in the application of molecular biology, 
    spectrophotometric, and chromatographic methods relevant to nutritional science
  • Be experienced and proficient in techniques of protein biochemistry and cell biology
  • Have knowledge of the qualitative and quantitative value of biochemical,
    cell-biological, molecular biological, and enzymatic analytical processes


Course contents

  • Primer design, PCR, cloning, use of restriction enzymes, ligation
  • Heterologous overexpression of eukaryotic genes, production of recombinant proteins
  • Purification with affinity chromatography, SDS-PAGE analysis
  • Enzyme kinetics
  • Inhibitor studies (linear and non- linear regression)
  • Photometric determination of riboflavin status (ERGAC) and hemoglobin
  • Determination of glutathione concentrations and total antioxidant capacity in biological material
  • 2-dimensional gel electrophoresis
  • Crystallization of proteins, x-ray diffraction analysis


Course format(s)

Seminar (30%), laboratory (70%)


Total workload

180 hours

   Credit points:  6 ECTS credits

Module composition:

A. Course total


Aa. Contact hours

60: seminar (20 hours), practical exercise (40 hours)

Ab. Preparation/revision

70: preparation (30 hours), follow-up study (40 hours)

B. Autonomous work

Small group work (20 hours)

C. Final module examination 


Assessment format(s) and contribution to final grade

Balancing exam format

Repeat exam format

Format: Written exam
Grade: 100%

Written exam

Duration in semesters


Summer semester, 2nd or 4th Master semester
1 semester

Seating capacity

60 maximum

Language of instruction

German and English, using separate groups (since this course is offered in parallel to Agrobiotechnology students)

Module guidance: see the semester posting   Schedule: see the lecture directory    Required reading: see the semester posting