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Virological and serological tests for small and wild ruminants

(SM: sample material; FD: frequency and duration of test; ‡ not accredited)

Border disease virus (BDV) and Pestiviruses in wild ruminants (deer)

Virus detection

  • Virus isolation (TC)

SM: blood with anticoagulant, swabs, organs
FD: daily/ 7-9 days

  • Genome detection (RT-PCR)

SM: blood with anticoagulant, swabs, organs
FD: daily/ 48h

Antibody detection

  • Serum neutralisation test (SNT; Border Disease)

SM: serum, 1 ml
FD: on demand/ 5-7 days

Small ruminant lentiviruses / Maedi-Visna & Caprine Arthritis and Encephalitis

Virus detection

  • Genome detection (RT-PCR)‡

SM: organs (lung, CNS)
FD: daily/ ca. 48h

Antibody detection

  • competitive ELISA

SM: serum, 1 ml
FD: daily/ ca. 24h

Coronavirus and Rotavirus/ Diarrhea

Virus detection

  • Virus particle detection (EM)

SM: faecal samples, organs
FD: daily/ ca. 48h

  • Genome detection (RT-PCR)

SM: faecal samples, organs
FD: daily/ ca. 48h

Malignant catarrhal fever, persistent infections / Ovine/Caprine herpesvirus 2 (OvHV-2/CpHV-2), Alcelaphine Herpesvirus 1 (AlHV-1)

Virus detection

  • Genome detection (PCR)

SM: Blood with anticoagulant, nasal/ conjunctival swabs, organs
FD: daily/ ca. 48h

  • Quantitative genome detection (Real-Time PCR)

SM: Blood with anticoagulant, nasal/ conjunctival swabs, organs
FD: on demand/ ca. 48h

Antibody detection

  • Serum neutralisation test (AlHV, cross reaction)

        cannot be offered at the moment

Parapoxvirus/ Ecthyma contagiosum, ORF

Virus detection

  • Virus particle detection (EM)

SM: skin scrapes
FD: daily/ ca. 48h

  • Genome detection (PCR)‡

SM: skin scrapes
FD: daily/ ca. 48h

Ovine pulmonary adenocarcinoma / Jaagsiekte sheep retrovirus (JSRV) 

Virus detection

  •  Genome detection (RT-PCR) ‡

       SM: lung, nasal swab

       FD: daily/ ca. 48h

Bovine Norovirus / Diarrhea

Virus detection

  •  Genome detection (RT-PCR)

       SM: faeces

       FD: daily/ ca. 48h

  • Virus particle detection (EM)

       SM: faeces

       FD: daily/ ca. 48h

Bluetongue Virus Serotyp 8 (BTV)

Virus detection

  •  Genome detection (RT-PCR)

       SM: blood with anticoagulant, organs

       FD: daily/ ca. 48h

Schmallenbergvirus (SBV)

Virus detection

  •  Genome detection (RT-PCR)

       SM: blood with anticoagulant, organs

       FD: daily/ ca. 48h

Borna Disease / BoDV

Virus detection

  •  Genome detection (RT-PCR)

       SM: organs (CNS)

       FD: daily/ ca. 48h

Antibody detection

  • Immunofluorescence test

SM: serum, CSF (0.5ml)

FD: Tue, Thu/ 24h

Papillomavirus / Papillomatosis

Virusnachweis

  • Virus particle detection (EM)

       SM: warts

       FD: daily/ ca. 48h

  • Genome detection (PCR)‡

       SM: warts

       FD: daily/ ca. 48h

  • Vaccine production

       SM: warts (at least. 1g / 0.4g per animal)

       FD: daily/ ca. 10-14 days