Virological and serological tests for cattle
(SM: sample material; FD: frequency and duration of test; ‡ not accredited)
Bovine viral diarrhea virus (BVDV)-1,-2 / bovine viral diarrhea/ Mucosal Disease (BVD/MD)
Virus detection
- Virus isolation (TC)
SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: daily/ 7 days
- Genome detection (RT-PCR)
SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: daily/ 48h
- Genome detection differentiation BVDV 1/ BVDV 2 (RT-PCR)‡
SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: daily/ 48h
- Quantitative genome detection (Real time-RT-PCR)
SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: on demand/ 48h
Antibody detection
- Serum neutralisation test (SNT BVDV1 + BVDV2)
SM: serum, 2ml
FD: Tue and Fri/ 3 days
Papillomavirus / papillomatosis
Virus detection
- Virus particle detection (EM)
SM: warts
FD: daily/ 48h
- Genome detection (PCR)‡
SM: warts
FD: daily/ 48h
- Vaccine production‡
SM: warts (at least 1g /0,4g per animal)
FD: daily/ ca. 10-14 days
Bovine Coronavirus (BoCoV), Rotavirus, Norovirus / Diarrhea
Virus detection
- Virus particle detection (EM)
SM: faecal samples, organs
FD: daily/ ca. 48h
- Genome detection (RT-PCR, BoCoV/Rotavirus‡)
SM: faecal samples, organs
FD: daily/ 48h
Bovine respiratory syncytial virus (BRSV)
Virus detection
- Virus isolation (TC)
SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 7-14 days
- Genome detection (RT-PCR)
SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT)
SM: serum, 1ml
FD: Tue and Fri/ 4-5 days
Parainfluenzavirus 3 (PI 3)/ Shipping fever
Virus detection
- Virus isolation (TC)
SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 7-14 days
- Genome detection (RT-PCR)
SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT)
SM: serum, 1ml
FD: Tue and Fri/ 4-5 days
Bovine herpesvirus 1 (BHV-1) / Infectious bovine rhinotracheitis/ pustular vulvovaginitis (IBR/IPV)
Virus detection
- Virus isolation (TC)
SM: blood with anticoagulant (ca. 10ml), nasal/ pharyngeal swabs, genital swabs, organs
FD: daily/ 7 days
- Genome detection (PCR)
SM: blood with anticoagulant (ca. 10ml), nasal/ pharyngeal swabs, genital swabs, organs
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT)
SM: serum, 1ml
FD: Tue and Fri/ 3 days
Bovine herpesvirus 2 (BHV-2) / bovine mammilitis
Virus detection
- Virus isolation (TC)
SM: skin scrapes, biopsy
FD: daily/ 7 days
- Virus particle detection (EM)
SM: skin scrapes, biopsy
FD: daily/ 48h
- Genome detection (PCR)‡
SM: skin scrapes, biopsy
FD: daily/ 48h
Bovine herpesvirus 5 (BoHV-5)
Virus detection
- Genome detection differentiation BHV1/ BHV5 (PCR)
SM: see BoHV-1
FD: daily/ 48h
Ovine/Caprine herpesvirus 2 (OvHV-2/CpHV-2), Alcelaphine herpesvirus 1 (AlHV-1) / Bovine malignant catarrhal fever
Virus detection
- Genome detection (PCR)
SM: Blood with anticoagulants, nasal/ conjunctival swabs, organs
FD: daily/ 48h
- Quantitative genome detection (Real Time PCR)
SM: Blood with anticoagulants, nasal/ conjunctival swabs, organs
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT, AlHV)
cannot be offered at the moment
Bluetongue Virus (BTV)/ Blue tongue disease
Virus detection
- Genome detection (RT-PCR)
SM: Blood with anticoagulants, organs
FD: daily/ 48h
Bovines parapoxvirus-1, -2 / bovine papular stomatitis(1)/ pseudocowpox (2)
Virus detection
- Virus particle detection (EM)
SM: skin scrapes, vesicle fluid
FD: daily/ 48h
- Genome detection (PCR)
SM: skin scrapes, vesicle fluid
FD: daily/ 48h
Bovine herpesvirus 4 (BoHV-4)
Virus detection
- Virus isolation (TC)
SM: nasal swabs, organs, blood with anticoagulants
FD: daily/ 7-14 days
- Genome detection (PCR)‡
SM: nasal swabs, organs, blood with anticoagulants
FD: daily/ 48h
Schmallenbergvirus (SBV)
Virus detection
- Genome detection (RT-PCR) ‡
SM: organs, blood with anticoagulants
FD: daily/ 48h