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Virological and serological tests for cattle

(SM: sample material; FD: frequency and duration of test; ‡ not accredited)

Bovine viral diarrhea virus (BVDV)-1,-2 / bovine viral diarrhea/ Mucosal Disease (BVD/MD)

Virus detection

  • Virus isolation (TC)

SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: daily/ 7 days

  • Genome detection (RT-PCR)

SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: daily/ 48h

  • Genome detection differentiation BVDV 1/ BVDV 2 (RT-PCR)‡

SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: daily/ 48h

  • Quantitative genome detection (Real time-RT-PCR)

SM: blood with anticoagulant (ca. 10ml), serum, nasal/ pharyngeal swabs, organs
FD: on demand/ 48h

Antibody detection

  • Serum neutralisation test (SNT BVDV1 + BVDV2)

SM: serum, 2ml
FD: Tue and Fri/ 3 days

Papillomavirus / papillomatosis

Virus detection

  • Virus particle detection (EM)

SM: warts
FD: daily/ 48h

  • Genome detection (PCR)

SM: warts
FD: daily/ 48h

  • Vaccine production‡

SM: warts (at least 1g /0,4g per animal)
FD: daily/ ca. 10-14 days

Bovine Coronavirus (BoCoV), Rotavirus, Norovirus / Diarrhea

Virus detection

  • Virus particle detection (EM)

SM: faecal samples, organs
FD: daily/ ca. 48h

  • Genome detection (RT-PCR, BoCoV/Rotavirus)

SM: faecal samples, organs
FD: daily/ 48h

Bovine respiratory syncytial virus (BRSV)

Virus detection

  • Virus isolation (TC)

SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 7-14 days

  • Genome detection (RT-PCR)

SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 48h

Antibody detection

  • Serum neutralisation test (SNT)

SM: serum, 1ml
FD: Tue and Fri/ 4-5 days

Parainfluenzavirus 3 (PI 3)/ Shipping fever

Virus detection

  • Virus isolation (TC)

SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 7-14 days

  • Genome detection (RT-PCR)

SM: nasal/ pharyngeal swabs, bronchoalveolar lavage, organs
FD: daily/ 48h

Antibody detection

  • Serum neutralisation test (SNT)

SM: serum, 1ml
FD: Tue and Fri/ 4-5 days

Bovine herpesvirus 1 (BHV-1) / Infectious bovine rhinotracheitis/ pustular vulvovaginitis (IBR/IPV)

Virus detection

  • Virus isolation (TC)

SM: blood with anticoagulant (ca. 10ml), nasal/ pharyngeal swabs, genital swabs, organs
FD: daily/ 7 days

  • Genome detection (PCR)

SM: blood with anticoagulant (ca. 10ml), nasal/ pharyngeal swabs, genital swabs, organs
FD: daily/ 48h

Antibody detection

  • Serum neutralisation test (SNT)

SM: serum, 1ml
FD: Tue and Fri/ 3 days

Bovine herpesvirus 2 (BHV-2) / bovine mammilitis

Virus detection

  • Virus isolation (TC)

SM: skin scrapes, biopsy
FD: daily/ 7 days

  • Virus particle detection (EM)

SM: skin scrapes, biopsy
FD: daily/ 48h

  • Genome detection (PCR)‡

SM: skin scrapes, biopsy
FD: daily/ 48h

Bovine herpesvirus 5 (BoHV-5)

Virus detection

  • Genome detection differentiation BHV1/ BHV5 (PCR)

SM: see BoHV-1
FD: daily/ 48h

Ovine/Caprine herpesvirus 2 (OvHV-2/CpHV-2), Alcelaphine herpesvirus 1 (AlHV-1) / Bovine malignant catarrhal fever

Virus detection

  • Genome detection (PCR)

SM: Blood with anticoagulants, nasal/ conjunctival swabs, organs
FD: daily/ 48h

  • Quantitative genome detection (Real Time PCR)

SM: Blood with anticoagulants, nasal/ conjunctival swabs, organs
FD: daily/ 48h

Antibody detection

  • Serum neutralisation test (SNT, AlHV)

cannot be offered at the moment

Bluetongue Virus (BTV)/ Blue tongue disease

Virus detection

  • Genome detection (RT-PCR)

SM: Blood with anticoagulants, organs
FD: daily/ 48h

Bovines parapoxvirus-1, -2 / bovine papular stomatitis(1)/ pseudocowpox (2)

Virus detection

  • Virus particle detection (EM)

SM: skin scrapes, vesicle fluid
FD: daily/ 48h

  • Genome detection (PCR)

SM: skin scrapes, vesicle fluid
FD: daily/ 48h

Bovine herpesvirus 4 (BoHV-4)

Virus detection

  • Virus isolation (TC)

SM: nasal swabs, organs, blood with anticoagulants
FD: daily/ 7-14 days

  • Genome detection (PCR)‡

SM: nasal swabs, organs, blood with anticoagulants
FD: daily/ 48h

Schmallenbergvirus (SBV)

Virus detection

  • Genome detection (RT-PCR) ‡

      SM: organs, blood with anticoagulants

      FD: daily/ 48h