Microarray, Super-SAGE & RNA-seq
Aim of this project is the characterization of pairing-dependently transcribed genes in the male with a focus on the identification of competence factors. These are genes by definition whose expression is essential for the induction of pairing and maturation processes in the female.
Overview of the RNAseq Data from Analysis of paired and unpairedS.mansoni, as well as
their gonads. (a) Multidimesional scaling (Replcates are shown in the same colour. (b)
Sample distance matrix; colour code is replicated as in (a,c). Hierarchical
clustering based on RPKM. Horizontal lines represent single genes. Yellow
indicates higher transkript amount, while blue stands for lower amount. bM,
paired Males (darkblue in a,b); sM, single Male (in a,b: dark blue); bT, Testes
of bM, (in a,b: light blue); sT, testes from sM(in a,b light blue) bF, paired
female (in a,b:red) sF, unpaired female(in a,b:rose); bO, ovarie from bF(a,b:
yellow); sO, ovarie from sF (in a,b;okker)
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