Project 4: Isolation of organs and tissue
Isolation of organs and tissue from schistosomes
Recently, we established a novel method for the isolation, purification, and enrichment of whole intact reproductive organs of adult Schistosoma mansoni providing RNA as well as proteins of sufficient amounts as basis for tissue-specific analyses1. qRT-PCR analyses comparing transcript levels of selected genes based on RNA obtained from gonads and whole worms revealed a significant influence of the pairing status on the gene expression in ovary and testis, which was not traceable in whole worms2. More recently, the organ isolation protocol was adapted for isolating vitellaria and vitelline cells for further molecular and physiological characterisation3. Currently, comparative sub-transcriptomic RNA-seq analyses employing ovaries and testes from paired and unpaired adult schistosomes were performed revealing tissue-specific and paring-dependent gene expression patterns within the gonads of S. mansoni4,5. Results of these analyses will accelerate the elucidation of differentiation processes of the reproductive organs and the involved signal transduction processes. This will lead to putative molecular targets for novel concepts to combat schistosomiasis. Future plans aim at further developing and improving the novel method for the isolation of specific tissues from other Schistosoma life stages as well as investigating its applicability on other trematodes.
1Hahnel S, Lu Z, Wilson RA, Grevelding CG, Quack T. (2013)PLoS Negl Trop Dis. 7(7):e2336.
2Hahnel S, Quack T, Parker-Manuel SJ, Lu Z, Vanderstraete M, Morel M, Dissous C, Cailliau K, Grevelding CG. (2014)
Front Genet. 5:170.
3Lu Z, Quack T, Hahnel S, Gelmedin V, Pouokam E, Diener M, Hardt M, Michel G, Baal N, Hackstein H, Grevelding CG. (2015)Int J Parasitol. 45(9-10):663-72.
4Lu Z, Sessler F, Holroyd N, Hahnel S, Quack T, Berriman M, Grevelding CG (2016) Gonad-specific and pairing-dependent gene expression in the blood fluke Schistosoma mansoni. Sci Rep. 6:31150.
5Lu, Z., Sessler, F., Holroyd, N., Hahnel, S., Quack, T., Berriman, M., Grevelding, C.G. (2017) A gene expression atlas of adult Schistosoma mansoni and their gonads. Scientific Data (in press) [further details can be obtained from "Schisto Databases", see "News July 2017"]