Inhaltspezifische Aktionen

A04 Sjaak Philipsen/ Thamar van Dijk

Repurposing CRISPR/Cas9 for targeted demethylation of the gamma-globin promoters

Reactivation of fetal globin expression is clinically relevant for beta-hemoglobinopathy patients. We will determine whether DNA methylation of the fetal gamma-globin gene promoters is involved in their suppression in adult erythropoiesis. To achieve local DNA demethylation, we will take advantage of CRISPR/Cas9 technology to direct TET methylcytosine dioxygenases to the gamma-globin gene promoters. In addition, we will study the interplay of DNA methylation with putative fetal globin modulating pharmacological compounds, focussing on inhibitors of protein arginine methyltransferases.

 

Name Sjaak Philipsen
Email
Telephone +31 10 7044282

 

Name T.B. van Dijk
Email   
Telephone +31 10 7044282


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