Virological and serological tests for horses
(SM: sample material; FD: frequency and duration of test; ‡ not accredited)
Equine infectious anemia virus (EIAV)
Virus detection
- Genome detection (RT-PCR)
SM: blood with anticoagulant (ca. 5ml), organs
FD: daily/ 48h
Antibody detection
- Immunoprecipitation (Coggins-Test)
SM: serum, 1ml
FD: daily/ 2-4 days
Equine arteritis virus (EAV)
Virus detection
- Virus isolation (TC)
SM: nasal/ pharyngeal/ conjunctival swabs, blood with anticoagulant (ca. 5ml), semen, organs
FD: daily/ ca. 7-14 days
- Genome detection (RT-PCR)
SM: nasal/ pharyngeal/ conjunctival swabs, blood with anticoagulant (ca. 5ml), semen, organs
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT)
SM: serum, ca. 1ml
FD: Tue and Fri/ 4-6 days
Equine influenza virus A/equi1 and A/equi2 / horse flu
Virus detection
- Genome detection (RT-PCR)
SM: blood with anticoagulants, nasal/ pharyngeal swabs, organs
FD: daily/ 48h
Antibody detection
- Hemagglutination inhibition assay (HIA)
SM: serum, ca. 1ml
FD: daily/ 2 days
Borna disease virus (BDV)
Virus detection
- Genome detection (RT-PCR)
SM: organs (eye, ZNS), CSF (at least 10ml)
FD: daily/ 48h
Antibody detection
- Immunofluorescence test
SM: serum, CSF 0.5-1ml
FD: Mon, Wed and Fri/ ca. 24h
Coronavirus and Rotavirus / Diarrhea
Virus detection
- Virus particle detection (EM)
SM: faecal samples, organs
FD: daily/ 48h
- Genome detection (RT-PCR‡)
SM: faecal samples, organs
FD: daily/ 48h
Tick borne encephalitis virus (TBEV)
Virus detection
- Genome detection (RT-PCR)
SM: blood with anticoagulant (ca. 2ml), CSF, ticks
FD: daily/ 48h
Antibody detection
- Serum neutralisation test (SNT)
SM: serum, 0.5-1 ml
FD: on demand/ 3 days
Equine herpes virus 1 and 4 (EHV-1/EHV-4) / equine abortion, rhinopneumonitis, EHM
Virus detection
- Virus isolation (TC)
SM: acute respiratory disease: nasal/ pharyngeal swabs, aborts: organs, plazenta, neurological disease: blood with anticoagulant (ca. 10ml), CSF, CNS material
FD: daily/ 7 days
- Genome detection (PCR)
SM: acute respiratory disease: nasal/ pharyngeal swabs, aborts: organs, plazenta, neurological disease: blood with anticoagulant (ca. 10ml), CSF, CNS material
FD: daily/ ca. 48h
Antibody detection
- Serum neutralisation test (SNT)
SM: serum, 2ml (EHV 1+4)
FD: Tue and Fri/ 3 days
Equine herpesvirus 2/5 (EHV-2/5) / equine cytomegalia, keratoconjunctivits
Virus detection
Virus isolation (TC)
SM: blood with anticoagulant (ca. 10ml), nasal/ pharyngeal/ conjunctival swabs
FD: daily/ ca. 10-12 days
- Genome detection (PCR; EHV-5‡)
SM: blood with anticoagulant (ca. 10ml), nasal/ pharyngeal/ conjunctival swabs
FD: daily/ ca. 48h
Equines Herpesvirus 3 (EHV-3)/ Equine coital exanthema
Virus detection
- Genome detection (PCR)‡
SM: genital swabs
FD: daily/ ca. 48h
Rabies vaccination check up
Antibody detection
- FAVN test
SM: serum, 1ml
FD: Monday and Wednesday/ 2 days
Papillomavirus / papillomatosis, equine sarcoid
Virus detection
- Virus particle detection (papillome, EM)
SM: warts
FD: daily/ 48h
- Genome detection (sarcoid, EM)‡
SM: warts
FD: daily/ 48h
- Vaccine production ‡
SM: warts (at least 1g)
FD: daily/ ca. 10-14 days
West-Nile-Virus (WNV)
Virus detection
- Genome detection (RT-PCR) ‡
SM: CSF, organs (CNS)
FD: daily/ ca. 48 h
Vaccination check up (RV, eq. influenza virus, EAV, EHV-1/4)
Antibody detection
- Serum neutralisation tests, HIA, FAVN test
SM: serum, at least 2ml
FD: see respective descriptions