Publications
103. Hughes J., Lang Ch. (2025) Preparation of phycocyanobilin (PCB) with and without 13C/15N isotope labeling for in vitro phytochrome autoassembly. In "Phytochromes: Methods and Protocols" Winkler A. (ed.). Methods in Molecular Biology, 2970, 51-67. doi: 10.1007/978-1-0716-4791-2_4
102. Nagano S., Guan K., von Stetten D., Chen P.-Y., Song C., Barends T., ..., Mancuso A., Heyne K., Hughes J. (2025) Pr and Pfr structures of plant phytochrome A. Nature Communications, 16, 5319. doi: 10.1038/s41467-025-60738-w
101. Nagano S., Song C., Rohr V., Mackintosh M., Hoang O., Kraskov A., Yang Y., Hughes J; Heyne K., Mroginski M.A., Schapiro I., Hildebrandt P. (2025) Integrated study of fluorescence enhancement in the Y176H variant of cyanobacterial phytochrome Cph1. Biochemistry, 64, 1348-1358. doi: 10.1021/acs.biochem.4c00687.
100. Hughes J., Nagano S., Guan K., von Stetten D. et al. (2024, preprint) Pr and Pfr structures of plant phytochrome A. Research Square. doi: 10.21203/rs.3.rs-4850459/v1
99. Chiso K., Yamashino T., Suzuki R., Gans t., Trogu S., Hughes J., Aoki S. (2024) Light responses during early day phases of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and PSEUDO-RESPONSE REGULATOR (PRR) homologous genes in the moss Physcomitrium patens. Photochem Photobiol. doi: 10.1111/php.14047
98. Gerland L, Diehl A, Erdmann N, Hiller M, Lang C, Teutloff C, Hughes J, Oschkinat H (2024) Changes in secondary structure upon Pr to Pfr transition in cyanobacterial phytochrome Cph1 detected by DNP NMR. Chemistry, e202402454. doi: 10.1002/chem.202402454
97. Hughes J, Winkler A (2024) New insight into phytochromes: connecting structure to function. Ann Rev Plant Biol, 75, 153-183. doi: 10.1146/annurev-arplant-070623-110636
96. Bell D, Lindemann F, Gerland L, Aucharova H, Klein A, Friedrich D, Hiller M, Grohe K, van Rossum B, Diehl A, Hughes J, Mueller LJ, Linser R, Miller AF, Oschkinat H (2024) Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects. J. Biomol. NMR, 78. doi: 10.1038/s41598-022-09169-x
95. Shenkutie S, Nagano S, Hughes J (2024) Expression, purification and crystallization of the photosensory module of phytochrome B (phyB) from Sorghum bicolor. Acta Crystallogr F 80:59-66. doi: 10.1107/S2053230X24000827
94. Yang Y, Stensitzki T, Lang C, Hughes J, Mroginski MA, Heyne K (2023) Ultrafast protein response in the Pfr state of Cph1 phytochrome. Photochem Photobiol Sci. 22:919-930. doi: 10.1007/s43630-023-00362-z
93. Escobar F, Kneip C, Michael N, Hildebrandt T, Tavraz N, Gartner W, Hughes J, Friedrich T, Scheerer T, Mroginski MA, ̈Hildebrandt P (2020) The lumi‑R intermediates of prototypical phytochromes. J. Phys. Chem. B, 124, 4044−4055 doi: 10.1021/acs.jpcb.0c01059
92. Trogu S, Ermert AL, Stahl F, Nogué F, Gans T, Hughes J (2020) Multiplex CRISPR/Cas9 mutagenesis of the phytochrome gene family in Physcomitrella. Plant Mol. Biol. doi: 10.1007/s11103-020-01103-x
91. Nagano S, Guan K, Shenkutie S, Feiler Ch, Weiss M, Kraskov A, Buhrke D, Hildebrandt P, Hughes J (2020) Structural insights into photoactivation and signalling in plant phytochromes. Nature Plants, 6, 581-588 doi: 10.1038/s41477-020-0638-y
90. Hughes J (2020) Phytochrome photoreceptors: Rapid response. eLife 2020;9:e57105. doi: 10.7554/eLife.57105
89. Sadeghi M, Balke J, Schneider C, Nagano S, Stellmacher J, Lochnit G, Lang C, Weise C, Hughes J, Alexiev U (2020) Transient deprotonation of the chromophore affects protein dynamics proximal and distal to the linear tetrapyrrole chromophore in phytochrome Cph1. Biochemistry 59:1051-1062. doi: 10.1021/acs.biochem.9b00967.
88. Ermert AL, Stahl F, Gans T, Hughes J (2019) Analysis of Physcomitrella phytochrome mutants via photo- and polarotropism. Methods Mol Biol. 2026: 225-236. doi: 10.1007/978-1-4939-9612-4_19.
87. Ermert AL, Nogué F, Stahl F, Gans T, Hughes J (2019) CRISPR/Cas9-mediated knockout of Physcomitrella patens phytochromes. Methods Mol Biol 2026: 237-263. doi: 10.1007/978-1-4939-9612-4_20.
86. Xu QZ, Bielytskyi P, Otis J, Lang C, Hughes J, Zhao KH, Losi A, Gärtner W, Song C (2019) MAS NMR on a red/far-red photochromic cyanobacteriochrome all2699 from Nostoc. Int. J. Mol. Sci. 20, 3656.
85. Song C, Mroginski MA, Lang C, Kopycki J, Gärtner W, Matysik J, Hughes J (2018) 3D structures of plant phytochrome A as Pr and Pfr from solid-state NMR: Implications for molecular function. Front Plant Sci 9: 498 doi: 103389/fpls201800498
84. Velázquez Escobar F, Buhrke D, Fernandez Lopez M, Shenkutie S, von Horsten S, Essen L-O, Hughes J, Hildebrandt P (2017) Structural communication between the chromophore binding pocket and the N-terminal extension in plant phytochrome phyB. FEBS Letters, 591:1258-1265 doi: 101002/1873-346812642
83. Stensitzki T, Yang Y, Wolke, AL, Knapp W, Hughes J, Mroginski MA, Heyne K (2017) Influence of heterogeneity on the ultrafast photoisomerization dynamics of Pfr in Cph1 phytochrome. Photochem Photobiol, 93:703-712 doi: 101111/php12743
82. Velázquez Escobar F, Lang C, Takiden A, Schneider C, Balke J, Hughes J, Alexiev U, Hildebrandt P, Mroginski MA (2017) Protonation-dependent structural heterogeneity in the chromophore binding site of cyanobacterial phytochrome Cph1. J Phys Chem B 121, 47-57, doi: 101021/acsjpcb6b09600
81. Stöppler D, Song C, van Rossum BJ, Geiger MA, Lang C, Mroginski MA, Jagtap AP, Sigurdsson ST, Matysik J, Hughes J, Oschkinat H (2016) Dynamic nuclear polarization provides new insights into chromophore structure in phytochrome photoreceptors. Angew Chem Intl Ed, 55, 16017-16020, doi: 101002/anie201608119
80. Ermert AL, Mailliet K, Hughes J (2016) Holophytochrome-interacting proteins in Physcomitrella: putative actors in phytochrome cytoplasmic signaling. Front Plant Sci 7, 613, doi: 103389/fpls201600613
79. Richter C, Schneider C, Quick MT, Volz P, Mahrwald R, Hughes J, Dick B, Alexiev U, Ernsting NP (2015) Dual-fluorescence pH probe for bio-labelling. Phys Chem Chem Phys,17, 30590-30597
78. Song C, Lang C, Kopycki J, Hughes J, Matysik (2015) NMR chemical shift pattern changed by ammonium sulfate precipitation in cyanobacterial phytochrome Cph1. Front Mol Biosci, 2, 42, doi: 103389/fmolb201500042
77. Velázquez Escobar F, von Stetten D, Günther-Lütkens M, Keidel A, Michael N, Lamparter T, Essen LO, Hughes J, Gärtner W, Yang Y, Heyne K, Mroginski MA, Hildebrandt P (2015) Conformational heterogeneity of the Pfr chromophore in plant and cyanobacterial phytochromes. Front Mol Biosci, 2, 37, doi: 103389/fmolb201500037
76. Johansson H, Hughes J (2014) Nuclear Phytochrome B regulates leaf flattening through Phytochrome Interacting Factors. Molecular Plant, 7, 1693-1696. doi: 101093/mp/ssu077
75. Sineshchekov V, Mailliet J, Psakis G, Feilke K, Kopycki J, Zeidler M, Essen L-O, Hughes J (2014) Tyrosine 263 in cyanobacterial phytochrome Cph1 optimizes photochemistry at the prelumi-R → lumi-R step. Photochem Photobiol, 90, 786-795, doi: 101111/php12263
74. Song C, Psakis G, Kopycki J, Lang C, Matysik J, Hughes J (2013) The D-ring, not the A-ring, rotates in Synechococcus OS-B' phytochrome. J Biol Chem, 289, 2552-2562, doi: 101074/jbcM113520031
73. Hughes J (2013) Phytochrome cytoplasmic signaling. Ann Rev Pl Biol, 64, 377-402, doi: 10.1146/annurev-arplant-050312-120045.
72. Song C, Rohmer T, Tiersch M, Zaanen J, Hughes J, Matysik J (2013) Solid-state NMR spectroscopy to probe photoactivation in canonical phytochromes. Photochem Photobiol, 89, 259–273
71. Jaedicke K, Lichtenthäler AL, Meyberg R, Zeidler M, Hughes J (2012) A phytochrome-phototropin light signaling complex at the plasma membrane. Proc Natl Acad Sci USA, 109, 12231-12236
70. Song C, Essen L-O, Gärtner W, Hughes J, Matysik J (2012) Solid-state NMR spectroscopic study of chromophore–protein interactions in the Pr ground state of plant phytochrome A. Mol Plant, 5, 698-715
69. Song C, Lang C, Mailliet J, Hughes J, Gärtner W, Matysik J (2012) Exploring chromophore-binding pocket: High-resolution solid-state 1H-13C interfacial correlation NMR spectra with windowed PMLG scheme. Applied Magnetic Resonance, 42, 79-88
68. Song C, Psakis G, Lang C, Mailliet J, Zaanen J, Gärtner W, Hughes J, Matysik J (2011) On the collective nature of phytochrome photoactivation. Biochemistry, 50, 10987-10989
67. Mailliet J, Psakis G, Feilke, K, Sineshchekov V, Essen L-O, & Hughes J (2011) Spectroscopy and a high-resolution crystal structure of Tyr-263 mutants of cyanobacterial phytochrome Cph1. J Mol Biol, 413, 115-127
66. Psakis G, Mailliet J, Lang C, Teufel L, Essen L-O, Hughes J (2011) Signalling kinetics of cyanobacterial phytochrome Cph1, a light regulated histidine kinase. Biochemistry, 50, 6178-6188
65. Jaedicke K, Rösler J, Gans T, Hughes J (2011) Bellis perennis - a useful tool for protein localization studies. Planta, 234, 759-768
64. Song C, Psakis G, Lang C, Mailliet J, Gärtner W, Hughes J, Matysik J (2011) Two ground state isoforms and a chromophore D-ring photoflip triggering extensive intramolecular changes in a canonical phytochrome. Proc Natl Acad Sci USA, 108, 15229–15234
63. Anders K, von Stetten D, Mailliet J, Kiontke S, Sineshchekov VA, Hildebrandt P, Hughes J, Essen L-O (2011) Spectroscopic and photochemical characterization of the red-light sensitive photosensory module of Cph2 from Synechocystis PCC 6803. Photochem Photobiol, 87, 160–173
62. Hughes J (2010) Physcomitrella: the green yeast (Book review: "The moss Physcomitrella patens", Knight C, Perroud PF & Cove D, Annual Plant Reviews vol 36, Wiley-Blackwell, Oxford) J Plant Physiol, 167, 1116-1117
61. Rohmer T, Lang C, Gärtner W, Hughes J, Matysik J (2010) Role of the protein cavity in chromoprotein assembly and double-bond isomerization in phytochrome: a comparison with model compounds. Photochem Photobiol, 86, 856-861
60. Röben M, Hahn J, Klein E, Lamparter T, Psakis G, Hughes J, Schmieder P (2010) NMR spectroscopic investigation of mobility and hydrogen bonding of the chromophore in the binding pocket of phytochrome proteins. ChemPhysChem, 11, 1248-1257
59. Hughes J (2010) On the structure and function of phytochromes, pp 7-24. In "Plant photobiology and photosynthesis", Volotovski ID, Ed, Nat Acad Sci Belarus, Minsk
58. Rohmer T, Lang C, Bongards C, Gupta S, Neugebauer J, Hughes J, Gärtner W & Matysik, J (2010) Phytochrome as molecular machine: Revealing chromophore action during the Pfr→Pr photoconversion by magic-angle spinning NMR. J Am Chem Soc, 132, 4431–4437
57. Hughes J (2010) Phytochrome 3D structures and functions. Biochem Soc Trans, 38, 710-716
56. Mailliet J, Psakis G, Schröder, C, Kaltofen S, Dürrwang U, Hughes J & Essen L-O (2009) Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins. Acta Crystallogr D, 65, 1232-1235
55. Mroginski MA, von Stetten D, Escobar FV, Strauss HM, Kaminski S, Scheerer P, Günther M, Murgida DH, Schmieder P, Bongards C, Gärtner W, Mailliet J, Hughes J, Essen L-O, Hildebrandt P (2009) Chromophore structure of cyanobacterial phytochrome Cph1 in the Pr state: reconciling structural and spectroscopic data by QM/MM calculations. Biophys J, 96, 4153-4163
54. Rohmer T, Lang C, Hughes J, Essen L-O, Gärtner W, Matysik J (2008) Light-induced chromophore activity and signal transduction in phytochromes observed by 13C and 15N magic-angle spinning NMR. Proc Natl Acad Sci USA, 105, 15229–15234
53. Essen L-O, Mailliet J, Hughes J (2008) The structure of a complete phytochrome sensory module in the Pr ground state. Proc Natl Acad Sci USA, 105, 14709–14714
52. Rensing et al [Hughes J as one of 70 co-authors] (2008) The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants. Science, 319, 64-69
51. Rohmer T, Strauss H, Hughes J, Gärtner W, de Groot H, Schmieder P, Matysik J (2006) 15N MAS-NMR studies of Cph1 phytochrome: chromophore dynamics and intramolecular signal transduction. J Phys Chem B, 110, 20580-20585
50. Zeidler M, Lang C, Hahn J, Hughes J (2006) Real-time spectral analysis during phytochrome chromophore and chromoprotein purification. Int J Biol Macromolecules, 39, 100-103
49. Hahn J, Strauss H, Landgraf F, Faus Gimenèz H, Lochnit G, Schmieder P, Hughes J (2006) Probing protein-chromophore interactions in Cph1 phytochrome via mutagenesis. FEBS J, 273,1415–1429
48. Suetsugu N, Mittmann F, Wagner G, Hughes J, Wada M (2005) A chimeric photoreceptor gene, NEOCHROME, has arisen twice during plant evolution. Proc Natl Acad Sci USA, 102, 13705-13709
47. Strauss HM, Schmieder P, Hughes J (2005) Light-dependent dimerisation in the N-terminal sensory module of cyanobacterial phytochrome 1. FEBS Lett, 579, 3970-3974
46. Strauss HM, Hughes J, Schmieder P (2005) Heteronuclear solution-state NMR studies of the chromophore in cyanobacterial phytochrome Cph1. Biochemistry, 44, 8244-8250
45. Hughes J, Brücker G, Repp A, Zeidler M, Mittmann F (2005) Phytochromes and functions: studies using gene targeting in Physcomitrella, pp 103-110. In "Light sensing in plants" Eds Wada M, Shimazaki K & Iino M Springer Verlag / Botanical Society of Japan (ISBN: 4-431-24002-0)
44. Mittmann F, Brücker G, Zeidler M, Repp A, Abts T Hartmann E, Hughes J (2004) Targetted knockout in Physcomitrella reveals direct actions of phytochrome in the cytoplasm. Proc Natl Acad Sci USA, 101, 13939-13944
43. Borucki B, Otto H, Rottwinkel G, Hughes J, Heyn M, Lamparter T (2003) Mechanism of Cph1 phytochrome assembly from stopped-flow kinetics and circular dichroism. Biochemistry, 42: 13684-97
42. Otto H, Lamparter T, Borucki B, Hughes J, Heyn M (2003) Dimerization and inter-chromphore distance of Cph1 phytochrome from Synechocystis, as monitored by fluorescence homo and hetero energy transfer. Biochemistry, 42, 5885-95
41. Lamparter T, Hughes J (2003) Phytochromes and phytochrome-like proteins in cyanobacteria, , pp 201 – 227. In "Photoreceptors and light signalling" Ed A Batschauer (Comprehensive Series in Photochemical and Photobiological Sciences, Eds Häder, Jori) Royal Society of Chemistry (ISBN 0-85404-311-X)
40. Hughes J (2003) Quo vadis Bachelor / Master? Laborjournal (07/08)
39. Sineshchekov V, Koppel' K, Esteban B, Hughes J, Lamparter T (2002) Fluorescence investigation of the recombinant cyanobacterial phytochrome (Cph1) and its C-terminally truncated monomeric species (Cph1Δ2): implication for holoprotein assembly, chromophore-apoprotein interaction and photochemistry. J Photochem Photobiol B, 67, 39-50
38. Heyne J, Herbst J, Stehlik D, Esteban B, Lamparter T, Hughes J, Diller R (2002) Ultrafast Dynamics of recombinant phytochrome from the cyanobacterium Synechocystis reconstituted with phycocyanobilin and phycoerythrobilin. Biophys J, 82, 1004-1016
37. Landgraf F, Forreiter C, Hurtado Picó A, Lamparter T, Hughes, J (2001) Recombinant holophytochrome in Escherichia coli. FEBS Letts, 508, 459-462
36. Lamparter T, Esteban B, Hughes, J (2001) Phytochrome Cph1 from the cyanobacterium Synechocystis PCC6803: purification, assembly, and quarternary structure. Eur J Biochem, 268, 4720-30
35. van Thor J, Borucki B, Lamparter T, Hughes J, Hellingwerf K, Heyn, M (2001) Light-induced proton release and -uptake in the cyanobacterial phytochrome, Cph1. Biochemistry, 40, 11460-71
34. Sineshchekov V, Hennig T, Lamparter T, Hughes J, Gärtner W, Schäfer E (2001) Recombinant phytochrome A in yeast differs by its spectroscopic and photochemical properties from the major phyA' and is close to the minor phyA'': evidence for post-translational modification of the pigment in plants. Photochem Photobiol, 73, 692-6
33. Briggs WR, Beck CF, Cashmore AR, Christie JM, Hughes J, Jarillo JA, Kagawa T, Kanegae H, Liscum E, Nagatani A, Okada K, Salomon M, Rüdiger W, Sakai T, Takano M, Wada M, Watson JC (2001) The phototropin family of photoreceptors. Plant Cell, 13, 993-997
32. Sineshchekov V Koppel’ L, Hughes J, Lamparter T, Zeidler M (2000) Recombinant phytochrome of the moss Ceratodon purpureus (CP2): fluorescence spectroscopy and photochemistry. J Photochem Photobiol, 56, 145-153
31. Foerstendorf H, Lamparter T, Hughes J, Gärtner W, Siebert F (2000) The photoreactions of recombinant phytochrome from the cyanobacterium Synechocystis: A low temperature UV-Vis and FT-IR spectroscopic study. Photochem Photobiol, 71, 655-661
30. Hughes J, Lamparter T (1999) Prokaryotes and phytochrome: the connection to chromophores and signaling. Plant Physiol, 121, 1059-1068
29. Hartmann E, Hughes J (1999) Photomorphogenesis of mosses: physiology and molecular biology, pp 49-77. In "Plant photobiology and photosynthesis", Volotovski ID, Ed Nat Acad Sci Belarus, Minsk
28. Zeidler M, Hartmann E, Hughes J (1999) Transgene expression in the moss Ceratodon purpureus. J Plant Physiol, 154, 641-650
27. Hughes J, Hartmann E (1999) Photomorphogenesis in lower plants, pp 805-837. in "Concepts in photobiology: photosynthesis and photomorphogenesis" Singhal G, Renger W, Sopory S, Irrgang K-D, Govindjee, Eds. Narosa Publishing,
26. Zeidler M, Lamparter T, Hughes J, Hartmann E, Remberg A, Braslavsky S, Schaffner K, , Gärtner W (1998) Recombinant phytochrome of the moss Ceratodon purpureus: Heterlogous expression and kinetic analysis of Pr Pfr conversion. Photochem Photobiol, 68, 857-863
25. Lamparter T, Hughes J, Hartmann E (1998) Blue light- and genetically-reversed gravitropic response in protonemata of the moss Ceratodon purpureus. Planta, 206, 95-102
24. Sineshchekov V, Hughes J, Lamparter, T (1998) Fluorescence and photochemistry of recombinant phytochrome from the cyanobacterium Synechocystis. Photochem Photobiol, 67: 263-267
23. Lamparter T, Brücker G, Esch H, Hughes J, Meister A, Hartmann E (1998) Somatic hybridisation with aphotopropic mutants of the moss Ceratodon purpureus: genome size, phytochrome photoreversibility, tip-cell phototropism and chlorophyll regulation. J Plant Physiol, 153: 394-400
22. Schneider-Poetsch HAW, Kolukisaoglu Ü, Clapham D, Hughes J, Lamparter T (1998) Non-angiosperm phytochromes and the evolution of vascular plants. Physiol Plantarum, 102, 612-622
21. Hughes J (1998) Molekulare und physiologische Ansätze zum Phytochromsystem. Habilitation thesis, Free University of Berlin
20. Remberg A, Lindner I, Lamparter T, Hughes J, Kneip K, Hildebrandt P, Braslavsky SE, Gärtner W, Schaffner, K (1997) Raman spectroscopic and light-induced kinetic characterization of a recombinant phytochrome of the cyanobacterium Synechocystis. Biochemistry, 36: 13389-13395
19. Lamparter T, Mittmann F, Gärtner W, Börner T, Hartmann E, Hughes J (1997) Characterization of recombinant phytochrome from the cyanobacterium Synechocystis. Proc Natl Acad Sci USA, 94: 11792-11797
18. Hughes J, Lamparter T, Mittmann F, Hartmann E, Gärtner W, Wilde A & Börner T (1997) A prokaryotic phytochrome. Nature, 386: 663, doi: 10.1038/386663a0
17. Hughes J (1996) pIDDLE6: A system for ligation and expression-cloning in E coli. Biotech Techniques, 10: 831-838
16. Lamparter T, Esch H, Cove D, Hughes J, Hartmann E (1996) Aphototropic mutants of the moss Ceratodon purpureus with spectrally-normal and with spectrally dysfunctional phytochrome. Plant, Cell, Environ, 19: 560-568
15. Hughes J, Lamparter T, Mittmann F (1996) Cerpu;PHY0;2, a "normal" phytochrome in Ceratodon (Accession No U56698) (PGR 96-067). Plant Physiol, 112: 446
14. Zeidler M, Gatz C, Hartmann E, Hughes J (1996) Tetracycline-regulated reporter gene expression in the moss Physcomitrella patens. Plant Mol Biol, 30: 199-205
13. Lamparter T, Podlowski S, Mittmann F, Schneider-Poetsch H A W, Hartmann E, Hughes J (1995) Phytochrome from protonemal tissue of the moss Ceratodon purpureus. J Plant Physiol, 147: 426-434
12. Lamparter T, Hughes J, Hartmann E (1994) A fully automated dual-wavelength photometer for phytochrome measurements and its application to phytochrome from chlorophyll-containing extracts. Photochem Photobiol, 60: 179-183
11. Bisztray G D, Hughes J, Qoronfleh M W (1994) Sequencing of double-stranded nucleic acid with thionucleotide triphosphates. BioTechniques, 16: 817-818
10. Hughes J, Qoronfleh M W, Bizstray G, Pratt L H (1993) PIDDLE: PCR-based, in-frame, directional DNA ligation and expression. Biotech Techniques, 7: 91-98
9. Hughes J, Qoronfleh M W (1991) Perspectives in plant genetic engineering and biopharmacy. Biopharmacy, 4: 18-28
8. Hughes J, Link G (1988) Photoregulation of psbA transcript levels in mustard cotyledons. Photosynth Res, 17: 57-73 Also in: "Molecular biology of photosynthesis", Govindjee, Bohnert, Bottomly, Bryant, Mullet, Ogren, Pakrasi, Somerville, Eds Kluwer, Dortrecht, pp 423-439
7. Hughes J, Neuhaus H, Link G (1987) Transcripts of two adjacent chloroplast genes during mustard (Sinapis alba L) seedling development are under differential temporal and light control. Plant Mol Biol, 9: 355-363
6. Hughes J, Wagner E (1987) Photomorphogenesis in Chenopodium album: effects of supplementary far-red light on stem extension kinetics. Planta, 172: 131-138
5. Hughes J, Beggs C J (1986) The dark side of sunlight - the facts about UV in sunlight. New Scientist, #1522 (August 21): 31-35
4. Hughes J, Morgan D C, Black C R (1985) Transmission properties of an oak canopy in relation to photoperception Plant, Cell, Environ, 8: 509-516
3. Hughes J, Heathcote L, Bambridge K, Black C R (1984) A growth cabinet providing variable spectral photon distributions at high fluence rates. New Phytol, 98: 211-219
2. Hughes J, Morgan D C, Lambton P A, Black C R, Smith H (1984) Photoperiodic time-signals during twilight. Plant, Cell, Environ, 7: 269-277
1. Hughes J (1983) Natural daylight and the simulation of its effects upon stomata. PhD Thesis, University of Nottingham