Projects
Synthetic small RNAs as tools for gene regulation
Fig. 1 Synthetic sRNAs (illustration: E.C. Bozcheloe)
Small RNAs (sRNAs) are versatile regulators in prokaryotes. In most cases, sRNAs consist of distinct modules, such as seed regions, binding sites for RNA-binding proteins, and Rho-independent terminator hairpins. Synthetic biology approaches will be used to recombine these modules and construct (semi-) synthetic sRNAs for gene regulation in bacteria. We will explore the potential of these synthetic sRNAs to control complex phenotypic traits, such as antibiotic resistance and production of biomaterials.
(Philipp, Brinkmann et al. (2023) Bioinformatics; Köbel, Melo Palhares, et al. (2022) ACS Synth Biol)