Inhaltspezifische Aktionen


Rager C, Klöpper T, Pfeil U, Tasch S, Whittaker M, Exintaris B, Mietens A, Middendorff R. (2023). Reference Gene U2 Enables Direct Comparison Between Relative Gene Expression Levels of Vascular Smooth Muscle Cells in Tissue and Culture Using Real-Time Quantitative PCR. Cells 12(17):2135


Humpfle L, Hachem NE, Simon P, Weinhold B, Galuska SP, Middendorff R. (2023). Knockout of the polysialyltransferases ST8SiaII and ST8SiaIV leads to a dilatation of rete testis during postnatal development. Front Physiol 14:1240296


Pleuger C, Ai D, Hoppe M, Winter LT, Bohnert D, Karl D, Guenther S, Epelman S, Kantores C, Fijak M, Ravens S, Middendorff R, Mayer JU, Loveland KL, Hedger M, Bhushan S, Meinhardt A. (2022). The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis. eLife 11:e82193


Seidensticker M, Tasch S, Mietens A, Exintaris B, Middendorff R. (2022). Treatment of benign prostatic hyperplasia and abnormal ejaculation: live imaging reveals tamsulosin - but not tadalafil - induced dysfunction of prostate, seminal vesicles and epididymis. Reproduction 164(6):291-301


Hachem NE, Humpfle L, Simon P, Kaese M, Weinhold B, Günther J, Galuska SP, Middendorff R (2021) The Loss of Polysialic Acid Impairs the Contractile Phenotype of Peritubular Smooth Muscle Cells in the Postnatal Testis. Cells 10(6):1347


Stadler B, Nowell CJ, Whittaker MR, Arnhold S, Pilatz A, Wagenlehner FM, Exintaris B, Middendorff R (2021) Physiological and pharmacological impact of oxytocin on epididymal propulsion during the ejaculatory process in rodents and men. The FASEB Journal 00:e21639


Lee SN, Kraska J, Papargiris M, Teng L, Niranjan B, Hammar J, Ryan A, Frydenberg M, Lawrentschuk N, Middendorff R, Ellem SJ, Whittaker M, Risbridger GP, Exintaris B (2021) Oxytocin receptor antagonists as a novel pharmacological agent for reducing smooth muscle tone in the human prostate. Scientific Reports 11:6352


Stadler B, Whittaker MR, Exintaris B, Middendorff R (2020) Oxytocin in the male reproductive tract; The therapeutic potential of oxytocin-agonists and -antagonists. Frontiers in Endocrinology 11:565731


Klein B, Bhushan S, Günther S, Middendorff R, Loveland KL, Hedger MP, Meinhardt A (2020) Differential tissue-specific damage caused by bacterial epididymo-orchitis in the mouse. Mol Hum Reprod 26(4):215-227


Weiser D, Mietens A, Stadler B, Ježek D, Schuler G, Middendorff R (2020) Contractions transport exfoliated epithelial cells through the neonatal epididymis. Reproduction 160:109-116


Klein B, Pant S, Bhushan S, Kautz J, Rudat C, Kispert A, Pilatz A, Wijayarathna R, Middendorff R, Loveland KL, Hedger MP, Meinhardt A (2019) Dexamethasone improves therapeutic outcomes in a preclinical bacterial epididymitis mouse model. Hum Reprod 34(7):1195-1205


Ehlert A, Budde U, Middendorff R, Manthei G, Kemmling A, Tiemann B (2018) Reintroduction of the rat for experimental subarachnoid hemorrhage with accelerated clot formation: A low mortality model with persistent clots as a precondition for studies in vasospasm. J Neurol Surg A Cent Eur Neurosurg 79(5):424-433


Elfgen V, Mietens A, Mewe M, Hau T, Middendorff R (2018) Contractility of the epididymal duct-function, regulation and potential drug effects. Reproduction 156(4)R125-R141


Reckmann AN, Tomczyk CU, Davidoff MS, Michurina TV, Arnold S, Müller D, Mietens A, Middendorff R (2018) Nestin in the epididymis is expressed in vascular wall cells and is regulated during postnatal development an in case of testosterone deficienca. PLoS One 13(6):e0194585


Steinfeld K, Beyer D, Mühlfeld C, Mietens A, Eichner G, Altinkilic B, Kampschulte M, Jiang Q, Krombach GA, Linn T, Weidner W, Middendorff R (2018) Low Testosterone in ApoE/LDL receptor double-knockout mice is associated with rarefied testicular capillaries together with fewer and smaller Leydig cells. Sci Rep 8(1)5424


Wijayarathna R, de Kretser DM, Meinhardt A, Middendorff R, Ludlow H, Groome NP, Loveland KA, Hedger MP (2018) Activin over-expression in the testis of mice lacking the inhibin α-subunit gene is associated with androgen deficiency and regression of the male reproductive tract. Mol Cell Endocrinol 470:188-198


Kügler R, Mietens A, Seidensticker M, Tasch S, Wagenlehner FM, Kaschtanow A, Tjahjono Y, Tomczyk CU, Beyer D, Risbridger GP, Exintaris B, Ellem SJ, Middendorff R (2018) Novel imaging of the prostate reveals spontaneous gland contraction and excretory durct quiescence together with different drug effects. FASEB J 32(3):1130-1138


Wijayarathna R, de Kretser DM, Sreenivasan R, Ludlow H, Middendorff R, Meinhardt A, Loveland KL, Hedger MP. (2018) Comparative analysis of activins A and B in the adult mouse epididymis and vas deferens. Reproduction 155(1):15-23


Lee SN, Chakrabarty B, Wittmer B, Papargiris M, Ryan A, Frydenberg M, Lawrentschuk N, Middendorff R, Risbridger GP, Ellem SJ, Exintaris B (2017) Age Related Differences in Responsiveness to Sildenafil and Tamsulosin are due to Myogenic Smooth Muscle Tone in the Human Prostate. Sci Rep 7(1):10150


Sokurenko Y, Ulyanova V, Zelenikhin P, Kolpakov A, Blokhin D, Müller D, Klochkov V, Ilinskaya O (2016) The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase. Bioinorg Chem Appl. 2016:4121960


Stammler A, Lüftner BU, Kliesch S, Weidner W, Bergmann M, Middendorff R, Konrad L (2016) Highly Conserved Testicular Localization of Claudin-11 in Normal and Impaired Spermatogenesis. PLoS One 11(8):e0160349


Steger K, Baumgart-Vogt E, Bergmann M, Brehm R, Chakraborty T, Konrad L, Linn T, Lüers G, Meinhardt A, Middendorff R, Paradowska-Dogan A, Rathke C, Renkawitz-Pohl R, Schagdarsurengin U, Weidner W (2016) Forschungsbericht: Molekulare Mechanismen männlicher Infertilität – Ergebnisse der Klinischen Forschergruppe KFO 181. J Reprod Med Endokrinol 13 (4):138–147


Michel V, Duan Y, Stoschek E, Bhushan S, Middendorff R, Young JM, Loveland KA, Kretser DM, Hedger MP, Meinhardt A (2016) Uropathogenic Escherichia coli cause fibrotic remodelling of the epididymis. J Pathol 240(1):15-24


Saboor F, Reckmann A, Tomczyk CUM, Peters D, Weissmann N, Kaschtanow A, Schermuly RT, Michurina TV, Enikolopov G, Müller D, Mietens A, Middendorff R (2016) Nestin-expressing vascular wall cells drive development of pulmonary hypertension. Eur Respir J 47:876-888


Kaese M, Galuska C, Simon P, Braun B, Cabrera-Fuentes H, Middendorff R, Wehrend A, Jewgenow K, Galuska S (2015) Polysialylation of the neural cell adhesion molecule NCAM takes place in granulosa cells during apoptotic processes of atretic tertiary follicles. FEBS J 282(23)4595:4606


Stammler A, Hau T, Bhushan S, Meinhardt A, Jonigk D, Lippmann T, Pilatz A, Schneider-Hüther I, Middendorff R (2015) Epididymitis: ascending infection restricted by segmental boundaries. Human Reprod 30(7):1557-1565


Bayat B, Tjahjono Y, Berghöfer H, Werth S, Deckmyn H, De Meyer SF, Sachs UJ, Santoso S (2015) Choline Transporter-Like Protein-2: New von Willebrand Factor-Binding Partner Involved in Antibody-Mediated Neutrophil Activation and Transfusion-Related Acute Lung Injury. Arterioscler Thromb Vasc Biol 35(7):1616-1622


Sokurenko Yu V, Zelenikhin PV, Ulyanova VV, Kolpakov AI, Müller D, Ilinskaya ON (2015) Identification of 2', 3'-cGMP as an intermediate of RNA catalytic cleavage by binase and evaluation of its biological action. Russ J of Bioorganic Chemistry 41(1):31-36


Simon P, Feuerstacke C, Kaese M, Saboor F, Middendorff R, Galuska SP (2015) Polysialylation of NCAM characterizes the proliferation period of contractile elements during postnatal development of the epididymis. PLoS One 10(3):e0123960


Dietze R, Shihan M, Stammler A, Konrad L, Scheiner-Bobis G (2015) Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells. Mol Cell Endocrinol 405:1-13


Borgers M, Wolter M, Hentrich A, Bergmann M, Stammler A, Konrad L (2014) Role of compensatory meiosis mechanisms in human spermatogenesis. Reproduction 148(3):315-20


Hänsch M, Simon P, Schön J, Kaese M, Braun BC, Jewgenow K, Göritz F, Küpper J, Ahmadvand N, Geyer R, Middendorff R, Müller K, Galuska SP (2014) Polysialylation of NCAM correlates with onset and termination of seasonal spermatogenesis in roe deer. Glycobiology 24(6):488-493


Mietens A, Tasch S, Stammler A, Konrad L, Feuerstacke C, Middendorff R (2014) Time-lapse imaging as a tool to investigate contractility of the epididymal duct - effects of cGMP signalling. PLoS One 9(3):e 92603


Pfeil U, Kuncova J, Brüggmann D, Paddenberg R, Rafiq A, Henrich M, Weigand MA, Schlüter KD, Mewe M, Middendorff R, Slavikova J, Kummer W (2014) Intrinsic vascular dopamine – a key modulator of hypoxia-induced vasodilation in splanchnic vessels. J Physiol 592(Pt8):1745-1756


Bartel J, Feuerstacke C, Galuska CE, Weinhold B, Gerardy-Schahn R, Geyer R, Münster-Kühnel A, Middendorff R, Galuska SP (2014) Laser microdissection of paraffin embedded tissue as a tool to estimate the sialylation status of selected cell populations. Anal Chem 86(5):2326-2331


Werler S, Demond H, Damm OS, Ehmcke J, Middendorff R, Gromoll J, Wistuba J (2014) Germ cell loss is associated with fading Lin28a expression in a mouse model for Klinefelter’s syndrome. Reproduction 147(3):253-264


Lang T, Hudemann C, Tchatalbachev S, Stammler A, Michel V, Aslani F, Bhushan S, Chakraborty T, Renz H, Meinhardt A (2014) Uropathogenic Escherichia coli modulates innate immunity to suppress Th1-mediated inflammatory responses during infectious epididymis. Infect Immun 82(3):1104-1111

Thong A, Müller D, Feuerstacke C, Mietens A, Stammler A, Middendorff R (2014) Neutral endopeptidase (CD10) is abundantly expressed in the epididymis and localized to a distinct population of epithelial cells - Its relevance for CNP degradation. Moll Cell Endocrinol 382(1):234-243


Skwirba M, Zakrzewicz A, Atanasova S, Wilker S, Fuch-Moll G, Müller D, Padberg W, Grau V (2014) Expression of nestin after renal transplantation in the rat. APMIS 122:1020-1031


Bayat B, Tjahjono Y, Sydykov A, Werth S, Hippenstiel S, Weissmann N, Sachs UJ, Santoso S (2013) Anti-human neutrophil antigen-3a induced transfusion-related acute lung injury in mice by direct disturbance of lung endothelial cells. Arterioscler Thromb Vasc Biol. 33(11):2538-2548


Werth S, Bayat B, Tjahjono Y, Eskandar J, Berghöfer H, Bein G, Sachs UJ, Santoso S (2013) Rapid enzyme-linked immunosorbent assay for the detection of antibodies against human neutrophil antigens -1a, -1b, and -1c. Transfusion 53(1):193-201


Stammler A, Müller D, Tabuchi Y, Konrad L, Middendorff R (2013) TGFßs modulate permeability of the blood-epididymis barrier in an In Vitro model. PLoS One 8(11):e80611


Lang T, Dechant M, Sanchez V, Wistuba J, Boiani M, Pilatz A, Stammler A, Middendorff R, Schuler G, Bhushan S, Tchatalbachev S, Wübbeling F, Burger M, Chakraborty T, Mallidis C, Meinhardt A (2013) Structural and Functional Integrity of Spermatozoa Is Compromised as a Consequence of Acute Uropathogenic E. coli-Associated Epididymitis. Biol Reprod 89(3):59


Stammler A, Meyer SS, Plant AR, Townsley BT, Becker A, Gleissberg S (2013) Duplicated STM-like KNOX I genes act in floral meristem activity in Eschscholzia californica (Papaveraceae). Dev Genes Evol 223(5):289-301


Ulm C, Saffarzadeh M, Mahavadi P, Müller S, Prem G, Saboor F, Simon P, Middendorff R, Geyer H, Henneke I, Bayer N, Rinné S, Lütteke T, Böttcher-Friebertshäuser E, Gerardy-Schahn R, Schwarzer D, Mühlenhoff M, Preissner KT, Günther A, Geyer R, Galuska SP (2013) Soluble polysialylated NCAM: a novel player of the innate immune system in the lung. Cell Mol Life Sci 70(19):3695-3708


Fecher-Trost C, Wissenbach U, Beck A, Schalkowsky P, Stoerger C, Doerr J, Dembek A, Simon-Thomas M, Weber A, Wollenberg P, Ruppert T, Middendorff R, Maurer HH, Flockerzi V (2013) The in vivo TRPV6 protein starts at a non-AUG triplet, decoded as methionine, upstream of canonical initiation at AUG. J Biol Chem 288(23):16629-16644


Simon P, Bäumner S, Busch O, Röhrich R, Kaese M, Richterich P, Wehrend A, Müller K, Gerardy-Schahn R, Mühlenhoff M, Geyer H, Geyer R, Middendorff R, Galuska SP (2013) Polysialic Acid Is Present in Mammalian Semen as a Post-translational Modification of the Neural Cell Adhesion Molecule NCAM and the Polysialyltransferase ST8SiaII. J Biol Chem 288(26):18825-18833


Lin CS, Xin Z, Namiki M, Albersen M, Muller D, Lue TF (2013) Direct androgen regulation of PDE5 gene or the lack thereof. Int J Impot Res 25(3):81-85


Lin C-S, Albersen M, Xin Z, Namiki M, Müller D, Lue TF (2013) Phosphodiesterase-5 expression and  function in the lower urinary tract: A critical review. Urology 81 (3):480-487


Budnik LT, Brunswig-Spickenheier B, Müller D (2013) The gonads and their magic bullet: lysophosphatidic acid. Physiological and toxicological functions of LPA in female and male reproductive systems. In: Hla T, Spiegel S, Moolenaar W, Chun J, eds. Lysophospholipid receptors: Signaling and Biochemistry. New Jersey: John Wiley & Sons; p. 569-586


Bayat B, Tjahjono Y, Werth S, Berghöfer H, Reil A, Kroll H, Sachs UJ, Santoso S (2012) Implication of transfected cell lines for the detection of alloantibodies against human neutrophil antigen-3. Transfusion 52(3):613-621


Friedrich RE, Holstein AF, Middendorff R, Davidoff MS (2012) Vascular wall cells contribute to tumourigenesis in cutaneous neurofibromas of patients with neurofibromatosis type 1. A comparative histological, ultrastructural and immunohistochemical study. Anticancer Res 32:2139-2158


Weissgerber P, Kriebs U, Tsvilovskyy V, Olausson J, Kretz O, Stoerger C, Mannebach S, Wissenbach U, Vennekens R, Middendorff R, Flockerzi V, Freichel M (2012) Excision of Trpv6 Gene Leads to Severe Defects in Epididymal Ca2+ Absorption and Male Fertility Much Like Single D541A Pore Mutation. J Biol Chem 287:17930-17941


Mietens A, Tasch S, Feuerstacke C, Eichner G, Volkmann J, Schermuly RT, Grimminger F, Müller D, Middendorff R (2012) Phosphodiesterase 5 (PDE5) inhibition, ANP and NO rapidly reduce epididymal duct contractions, but long-term PDE5 inhibition in vivo does not. Mol Cell Endocrinol 349:145-153

Müller D, Mukhopadhyay AK, Davidoff MS, Middendorff R (2011) Cyclic GMP signaling in rat urinary bladder, prostate and epididymis: Tissue-specific changes with aging and in response to Leydig cell depletion. Reproduction 142:333-343


Volkmann J, Müller D, Feuerstacke C, Kliesch S, Bergmann M, Mühlfeld C, Middendorff R (2011) Disturbed spermatogenesis associated with thickened lamina propria of seminiferous tubules is not caused by dedifferentiation of myofibroblasts. Hum Reprod 26:1450-146

Weissgerber P, Kriebs U, Tsvilovskyy V, Olausson J, Kretz O, Stoerger C, Vennekens R, Wissenbach U, Middendorff R, Flockerzi V, Freichel M (2011) Male Fertility Depends on Ca2+ Absorption by TRPV6 in Epididymal Epithelia. Science Signaling 4 (171):ra27


Müller D, Hildebrand M, Lübberstedt J, Kuhn M, Middendorff R (2010) The membrane receptors guanylyl cyclase-A (GC-A) and GC-B undergo distinctive changes in posttranslational modification during brain development. J Neurochem 115:1024-1034


Sager H, Middendorff R, Rauche K, Weil J, Lieb W, Schunkert H, , Ito WD (2010) Temporal patterns of blood flow and nitric oxide synthase expression affect macrophage accumulation and proliferation during collateral growth. J Angiogenesis Res 2:18


Kota V, Rai P, Weitzel J, Middendorff R, Bhande S, Shivaji S (2010) Role of Glycerol-3-phosphate dehydrogenase 2 in mouse sperm capacitation. Mol Reprod Dev 77:773-783


Müller D, Greenland KJ, Speth RC, Middendorff R (2010) Neuronal differentiation of NG108-15 cells has impact on nitric oxide- and membrane (natriuretic peptide receptor-A) cyclic GMP-generating proteins. Moll Cell Endocrinol 320(1-2):118-127


Müller D, Hida B, Guidone G, Speth RC Michurina T, Enikolopov G, Middendorff R (2009) Expression of guanylyl cyclase (GC)-A and GC-B during brain development: Evidence for a role of GC-B in perinatal neurogenesis. Endocrinology 150:5520-5529


Davidoff MS, Middendorff R, Müller D, Holstein AF (2009) The Neuroendocrine Leydig Cells and their Stem Cell Progenitors, the Pericytes. Adv Anat Embryol Cell Biol 205:1-154


Schlosser G, Awtry T, Brugmann SA, Jensen ED, Neilson K, Ruan G, Stammler A, Voelker D, Yan B, Zhang C, Klymkowsky MW, Moody SA (2008) Eya1 and Six1 promote neurogenesis in the cranial placodes in a SoxB1-dependent fashion. Developmental Biology 320(1):199-214


Schermuly RT, Stasch JP, Pullamsetti SS, Middendorff R, Müller D, Schlüter KD, Dingendorf A, Kolosionek E, Hackemack S, Kaulen C, Medebach T, Dumitrascu R, Weissmann N, Mittendorf J, Klepetko W, Seeger W, Ghofrani HA, Grimminger F (2008) Expression and function of soluble guanylate cyclase in pulmonary arterial hypertension. Eur Respir J 32:881-891


Mewe M, Wulfsen I, Schuster A, Middendorff R, Glassmeier G, Schwarz JR, Bauer CK (2008) Erg K+ channels modulate contractile activity in the bovine epididymal duct. Am J Physiol Regul Integr Comp Physiol  294:R895-R904


Mewe M, Wulfsen I, Middendorff R, Bauer CK (2007) Differential modulation of bovine epididymal activity by oxytocin and noradrenaline. Reproduction 134:493-501


Weidtmann K, Harendza S, Dietsche S, Guse AH, Middendorff R, Schultz JH, Mihalache I, Frost M, Kuhnigk O (2007) Concept and evaluation of the integrated seminars during pre-clinical studies („Medicine I”) at Hamburg Medical School. GMS Z Med Ausbild


Nakaya N, Hemish J, Krasnov P, Kim SY, Stasiv Y, Michurina T, Herman D, Davidoff MS, Middendorff R, Enikolopov G (2007) Noxin, a novel stress-induced gene involved in cell cycle and apoptosis. Mol Cell Biol 27:5430-5444


Kuhnigk O, Weidtmann K, Dietsche S, Guse AH, Mihalache I, Schultz JH, Middendorff R, Kadula H, Harendza S (2006) The new pre-clinical curriculum “Medicine 1” at Hamburg Medical School. Curricular changes according to the new education law (ÄAppO). GMS Z Med Ausbild 23(2):Doc36


Mewe M, Bauer C, Schwarz JR, Middendorff R (2006) Mechanisms regulating spontaneous contractions in the bovine epididymal duct. Biol Reprod 75:651-659


Müller D, Cortes-Dericks L, Budnik LT, Brunswig-Spickenheier B, Mukhopadhyay AK, Middendorff R (2006) Homologous and lysophosphatidic acid (LPA)-induced desensitization of the atrial natriuretic peptide receptor, guanylyl cyclase-A, in MA-10 Leydig cells: Endocrinology 147:2974-2985


Müller D, Davidoff MS, , Bargheer O, Paust HJ, Pusch W, Koeva Y, Ježek D, Holstein AF, Middendorff R (2006) The expression of neurotrophins and their receptors in prenatal and adult human testes: Evidence for functions in Leydig cells. Histochem Cell Biol 126:199-211


Mewe M, Bauer CK, Müller D, Middendorff R (2006) Regulation of spontaneous contractile activity in the bovine epididymal duct by cyclic GMP-dependent pathways. Endocrinology 147:2051 - 2062


Davidoff MS, Ungefroren H, Middendorff R, Koeva Y, Bakalska M, Atanassova N, Holstein AF, Ježek D, Pusch W, Müller D (2005) Catecholamine-synthesising enzymes in the adult and developing human testis. Histochem Cell Biol 124:313-323


Davidoff MS, Middendorff R, Enikolopov G, Riethmacher D, Holstein AF, Müller D (2004) Progenitor cells of the testosterone-producing Leydig cells revealed. J Cell Biol 167:935-944


Rajković M, Middendorff R, Wetzel MG, Frković D, Damerow S, Seitz HJ, Weitzel HJ (2004) Germ cell nuclear factor (GCNF) relieves cAMP-response-element modulator tau (CREMtau)-mediated activation of the testis-specific promoter of human mitochondrial glycerol-3-phosphate dehydrogenase. J Biol Chem 279:52493-52499


Müller D, Mukhopadhyay AK, Speth RC, Guidone G, Potthast R, Potter LR, Middendorff R (2004) Spatiotemporal regulation of the two atrial natriuretic peptide receptors in testis. Endocrinology 145: 1392-1401


Willipinski-Stapelfeldt W, Lübberstedt J, Stelter S, Vogt K, Mukhopadhyay AK, Müller D (2004) Comparative analysis between cyclic GMP and cyclic AMP signalling in human spermatozoa. Mol Hum Reprod 10: 543-552


Olcese J, Domagalski R, Bednorz A, Weaver DR, Urbanski HF, Reuss S, Middendorff R (2003) Expression and regulation of mPer1 in immortalized GnRH neurons. Neuroreport 14: 613-618


Schlabritz-Loutsevitch N, Hellner N, Middendorff R, Müller D, Olcese J (2003) The human myometrium as a target for melatonin. J Clin Endocrinol Metab 88:908-913


Saric T, Müller D, Seitz H-J, Pavelic K (2003) Non-covalent interaction of ubiquitin with insulin-degrading enzyme. Mol Cell Endocrinol 204:11-20


Weitzel JM, Shiryaewa NB, Middendorff R, Balvers M, Radtke C, Ivell R, Seitz HJ (2003) Testis-specific expression of rat mitochondrial glycerol-3-phosphate dehydrogenase in haploid male germ cells. Biol Reprod 68: 699-707


Olcese J, Majora C, Stephan A, Müller D (2002) Nocturnal accumulation of cyclic GMP in the chick pineal organ is dependent on autocrine activation of guanylyl cyclase-B. J Neuroendocrinol 14:14-18


Guidone D, Müller D, Vogt K, Mukhopadhyay AK (2002) Characterization of VIP and PACAP receptors in cultured rat penis corpus cavernosum smooth muscle cells and their interaction with guanylate cyclase-B receptors. Regul Pept 108: 63-72


Behrends S, Mietens A, Kempfert J, Koglin M, Scholz H, Middendorff R (2002) The expression pattern of nitric oxide sensitive guanylyl cyclase in the rat heart changes during postnatal development. J Histochem Cytochem 50:1325-1331


Middendorff R, Müller D, Mewe M, Mukhopadhyay AK, Holstein AF, Davidoff MS (2002) The tunica albuginea of the human testis is characterized by complex contraction and relaxation activities regulated by cGMP. J Clin Endocrinol Metab 87:3486-3499


Davidoff MS, Middendorff R, Köfüncü E, Müller D, Jezek D, Holstein AF (2002) The Leydig cells of the human testis possess astrocyte and oligodendrocyte marker molecules. Acta Histochem 104: 39-49


Müller D, Middendorff R, Olcese J, Mukhopadhyay AK (2002) Central nervous system-specific glycosylation of the type A natriuretic peptide receptor. Endocrinology 143: 23-29


Ivell R, Kimura T, Müller D, Augustin K, Abend N, Bathgate RAD, Telgmann R, Balvers M, Tillmann G, Fuchs A-R (2001) The structure and regulation of the oxytocin receptor. Exp Physiol 86.2: 289-296


Einspanier A, Müller D, Lübberstedt J, Bartsch O, Jurdzinski A, Fuhrmann K, Ivell R (2001) Characterization of relaxin binding in the uterus of the marmoset monkey.

Mol Human Reprod 7: 963-970


Behrends S, Kempfert J, Mietens A, Koglin M, Scholz H, Middendorff R (2001) Developmental changes of nitric oxide sensitive guanylyl cyclase expression in pulmonary arteries. Biochem Biophys Res Commun 283: 883-887


Davidoff MS, Middendorff R, Koeva Y, Pusch W, Jezek D, Müller D (2001) Glial cell line-derived neurotrophic factor (GDNF) and its receptors GFRa-1 and GFRa-2 in the human testis. In: Molecular, cellular and developmental reproduction (Motta PM, Nottola SA, Macchiarelli G, Correr S) It J Anat Embryol 106 (suppl 2): 173-180


Davidoff MS, Middendorff R (2000) The nitric oxide system in the urogenital tract. In: Functional neuroanatomy of the nitric oxide system (Steinbusch HWM, De Vente J, Vincent SR, Björklund A, Hökfelt T, eds., Elsevier, Amsterdam) Handbook of Chemical Neuroanatomy, Vol. 17, p. 267-314


Middendorff R, Kumm M, Davidoff MS, Holstein AF, Müller D (2000) Generation of cyclic guanosine monophosphate (cGMP) by heme oxygenases in the human testis - A regulatory role for carbon monoxide (CO) in Sertoli cells? Biol Reprod 63: 651-657


 Middendorff R, Davidoff MS, Behrends S, Mewe M, Mietens A, Müller D (2000) Multiple roles of the messenger molecule cGMP in testicular function. Andrologia 32: 55-59


Müller D, Olcese J, Mukhopadhyay A, Middendorff R (2000) Guanylyl cyclase B represents the predominant natriuretic peptide receptor expressed at exceptionally high levels in the pineal gland. Mol Brain Res 75: 321-329


Müller D, Middendorff R, Davidoff MS (1999) Neurotrophic factors in the testis. Biomed Rev 10: 25-30


Davidoff MS, Middendorff R, Pusch W, Müller D, Wichers S, Holstein AF (1999) Sertoli cells and Leydig cells of the human testis express neurofilament triplet proteins. Histochem Cell Biol 111: 173-188


Nitz B, Ivell R, Hartung S, Middendorff R, Olcese J (1998) A fusca gene homologue in mammalian tissues: developmental regulation in the rat testes. Mol Cell Endorinol 144: 131-138


Wiesner B, Weiner J, Middendorff R, Hagen V, Kaupp UB, Weyand I (1998) Cyclic nucleotide-gated channels on the flagellum control Ca2+ entry in sperm. J Cell Biol 142: 473-484


Zumkeller W, Müller D, Müller S, Günther C, Westphal M, (1998) Expression and synthesis of insulin-like growth factor-binding proteins in human glioma cell lines. Int J Oncol 12: 129-135


Olcese J, McArdle CA, Middendorff R, Greenland K (1997) Pituitary adenylate cyclase-activating peptide (PACAP) and vasoactive intestinal peptide (VIP) receptor expression in immortalized LHRH neurons. J Neuroendocrinol 9: 937-943


Müller D, Paust HJ, Middendorff R, Davidoff MS (1997) Nerve growth factor (NGF) receptors in male reproductive organs. Adv Exp Med Biol 424: 157-158


Middendorff R, Müller D, Wichers S, Holstein AF, Davidoff MS (1997) Evidence for production and functional activity of nitric oxide (NO) in seminiferous tubules and blood vessels of the human testis. J Clin Endocrinol Metab 82: 4154-4161


Müller D, Middendorff R (1997) A new role of atrial natriuretic peptide (ANP) in the testis. Adv Exp Med Biol 424: 155-156


Middendorff R, Paust HJ, Davidoff MS, Olcese J (1997) Synthesis of C-type natriuretic peptide (CNP) by immortalized LHRH cells. J Neuroendocrinol 9: 177-182


Middendorff R, Müller D, Holstein AF (1997) The C-type natriuretic peptide (CNP) system in the testis: a physiological role of neuroactive factors in Leydig cells. Biomed Rev 8: 111-117


Maronde E*, Middendorff R*, Telgmann R, Müller D, Hemmings B, Tasken K, Olcese J (1997) Melatonin synthesis in the bovine pineal gland is regulated by type II cyclic AMP-dependent protein kinase. J Neurochem 68: 770-777

*The first two authors contributed equally to this work.


Middendorff R, Müller D, Paust HJ, Holstein AF, Davidoff MS (1997) New aspects of Leydig cell function. Adv Exp Med Biol 424: 125-138


Davidoff MS, Middendorff R, Mayer B, Koesling D, Holstein AF (1997) Nitric oxide/cGMP pathway components in Leydig cells of the human testis. Cell Tissue Res 287: 161-170


Davidoff MS, Middendorff R, Müller D, Köfüncü E, Holstein AF (1997) Immunoreactivity for glial cell markers in Leydig cells of the human testis. Adv Exp Med Biol 424: 151-152


Davidoff MS, Middendorff R, Holstein AF (1996) Dual nature of Leydig cells of the human testis. Biomed Rev 6:11-41


Böningk W, Müller F, Middendorff R, Weyand I, Kaupp UB (1996) Two alternatively spliced forms of the cGMP-gated channel a-subunit from cone photoreceptor are expressed in the chick pineal organ. J Neurosci 16: 7458-7468


Olcese J, Middendorff R, Maronde E, Paust HJ, Davidoff MS (1996) C-type natriuretic peptide (CNP) in the mammalian pineal gland: An endogenous autocrine peptide? In: The peptidergic neuron (Krisch B, Mentlein R, eds., Birkhäuser-Verlag, Basel) p. 297-302


Middendorff R, Müller D, Paust HJ, Davidoff MS, Mukhopadhyay AK (1996) Natriuretic peptides in the human testis: Evidence for a potential role of C-type natriuretic peptide (CNP) in Leydig cells. J Clin Endocrinol Metab 81: 4324-4328


Middendorff R*, Maronde E*, Paust HJ, Davidoff MS, Olcese J (1996) Expression of C-type natriuretic peptide (CNP) in the bovine pineal gland. J Neurochem 67: 517-524

*The first two authors contributed equally to this work.


Middendorff R, Davidoff MS, Mayer B, Holstein AF (1995) Neuroendocrine characteristics of human Leydig cell tumors. Andrologia 27: 351-355


Maronde E*, Middendorff R*, Mayer B, Olcese J (1995) The effect of NO-donors in bovine and rat pineal cells. J Neuroendocrinol 7: 207-214

*The first two authors contributed equally to this work.


Davidoff M, Middendorff R, Mayer B, Holstein AF (1995) Nitric oxide synthase (NOS-I) in Leydig cells of the human testis. Arch Histol Cytol 58: 17-30


McArdle CA, Olcese J, Schmidt C, Poch A, Kratzmeier M, Middendorff R (1994) C-type natriuretic peptide (CNP) in the pituitary: Is CNP an autocrine regulator in gonadotropes? Endocrinology 135: 2794-2801


Olcese J, Middendorff R, Münker M, Schmidt C, McArdle CA (1994) Natriuretic peptides stimulate cyclic GMP production in an immortalized LHRH neuronal cell line. J Neuroendocrinol 6: 127-130


McArdle CA, Ivell R, Kratzmeier M, Schmidt C, Müller D, Käppler K, Poch A (1994) Production and action of C-type natriuretic peptide in the gonadotrope-derived a-T3-1 cell line. Endocrine 2: 849-856


Olcese J, Müller D, Münker M, Schmidt C (1994) Natriuretic peptides elevate cyclic 3’, 5’-guanosine monophosphate levels in cultured rat pinealocytes: evidence for guanylate cyclase-linked membrane receptors. Mol Cell Endocrinol 103: 93-100


Middendorff R, Davidoff M, Holstein AF (1993) Neuroendocrine marker substances in human Leydig cells - changes by disturbances of testicular function. Andrologia 25: 257-262


Davidoff M, Schulze W, Middendorff R, Holstein AF (1993) The Leydig cell of the human testis - a new member of the neuroendocrine system. Cell Tissue Res 271: 429-439


Dautzenberg F, Müller D, Richter D (1993) Dephosphorylation of phosphorylated atrial natriuretic peptide by protein phosphatase 2A. Eur J Biochem 211: 485-490


Baumeister H, Müller D, Rehbein M, Richter D (1993) The rat insulin-degrading enzyme: molecular cloning and characterization of tissue-specific transcripts. FEBS Lett 317: 250-254


Schumacher H, Müller D, Mukhopadhyay AK (1992) Stimulation of testosteron production by atrial natriuretic peptide in isolated mouse Leydig cells results from a promiscuous activation of cyclicAMP-dependent protein kinase by cyclic GMP.

Mol Cell Endocrinol 90: 47-52


Müller D, Schulze C, Baumeister H, Buck F, Richter D (1992) The rat insulin-degrading enzyme: cleavage pattern of the natriuretic peptide hormones ANP, BNP and CNP revealed by HPLC and mass spectrometry. Biochemistry 31: 11138-11143


Müller D, Rehbein M, Baumeister H, Richter D (1992) Molecular characterization of a novel rat protein structurally related to poly (A) binding proteins and the 70K protein of the U1 small nuclear ribonucleoprotein particle (snRNP). Nucl Acids Res 20: 1471-1475


Müller D, Baumeister H, Buck F, Richter D (1991) Atrial natriuretic peptide (ANP) is a high-affinity substrate for rat insulin-degrading enzyme. Eur J Biochem 202: 285-292


Pirke KM, Schweiger U, Strowitzki T, Tuschl R, Laessle RG, Broocks A, Huber B, Middendorff R (1989) Dieting causes menstrual irregulations in normal weight young women through impairment of episodic luteinizing hormone secretion. Fertil Steril 51: 263-268


Hofer B, Müller D, Köster, H (1987) E. coli RNA polymerase binding to a DNA terminus prevents formation of a closed promoter complex. Biochim Biophys Acta 910: 254-260


Hofer B, Müller D, Köster H (1985) The pathway of E. coli RNA polymerase-promoter complex formation as visualized by footprinting. Nucl Acids Res 13: 5995-6013


Müller D, Hofer B, Koch A, Köster H (1983) Aspects of the mechanism of acid-phenol extraction of nucleic acids. Biochim Biophys Acta 740: 1-7


Frank R, Müller D, Wolff C (1981) Identification and suppression of secondary structures formed from deoxy-oligonucleotides during electrophoresis in denaturing polyacrylamid gels. Nucl Acids Res 9: 4967-4979


Dahlmann N and Müller D (1980) Thymidin-5’-triphosphatase: a new serum enzyme activity and its significance in malignant diseases. Hoppe Seyler’s Z  Physiol  Chem 361: 1851-1854