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Flux Balance Analysis and Flux Variability Analysis

Flux Balance Analysis (FBA) and Flux Variability Analysis (FVA)

Flux Balance Analysis (FBA) is a linear programming technique that uses metabolic models to predict phenotypic responses under given environmental conditions. It is a leading method for simulating and manipulating cellular growth in silico.
Flux Variability Analysis (FVA) is a widely used computational tool to determine the minimum and maximum ranges of reaction fluxes that satisfy the model constraints. It achieves this by solving a double linear programming problem, involving a maximization followed by a minimization for each reaction of interest.

Materials

 

Recommended Literature

What is flux balance analysis?, Orth et al. (2010)

Constraining the metabolic genotype-phenotype relationship using a phylogeny of in silico methods, Lewis et al. (2012)

Escher-FBA: a web application for interactive flux balance analysis, Rowe et al. (2018)

Tools

COBRApy

COBRA Toolbox