Inhaltspezifische Aktionen

News & Activities - AG Schetelig

2024-02 | Fruit Fly News features REACT

Fruit Fly News (FFN) is a Newsletter for Tephritid fruit fly workers around the world. It showcases current developments, news, and publications in the field, and introduces scientists and their contributions to the fruit fly research.

The current FFN Issue (#47, February 2024) presents the REACT initiative, and its goal to safeguard European agriculture from the invasive fruit flies Bactrocera zonata and Bactrocera dorsalis (p. 11). Furthermore, Andrea Moyano, Postdoc at the Institute of Molecular Biology of the National Research Council in Pavia, Italy, and part of the REACT team, shares her story in the “young scientists’ corner” (p. 17).

2024-01 |  Habilitation Dr. Ying Yan

With the habilitation thesis entiteled "Synthetic biology for functional genomics and pest control of insects", the presentation and colloquium for the theme "Invasion biology: a common theme for diverse taxa and changing environments", an inaugural lecture "How to transform an insect", and a party with colleagues and friends, Dr. Ying Yan is officially habilitated at the JLU with the Venia legendi for "Synthetic Biology in Insects". 

Upon receiving the Habilitation title, Dr. Yan expressed his tremendous appreciation to all colleagues at the IBPP who have been incredibly inspiring and supportive. He also extended deep gratitude to the academic council of Faculty 09 for considering and processing this application.

2023-12 |  Successful defense of PhD thesis

PhD student Germano Sollazzo successfully presented and defended his PhD thesis entiteled "Characterization of genes used as selectable markers for the development of genetic sexing strains for SIT applications against tephritid pest species using CRISPR/Cas9".

The cumulative thesis aims to identify the molecular background of the famous temperature-sensitive lethal phenotype in medfly, and includes three studies. In the first study, the temperature sensitivity of different medfly strains was assessed (published in Insects), in the second one, the putative genomic location of the tsl gene characterized (published in G3), and the third one, a potential tsl candidate gene analyzed. 

Congratulations, Dr. Sollazzo!

2023-11 |  Meeting on crop protection in Rome

The Accademia Nazionale Dei Lincei invited several scientists to their beautiful ancient buildings in Rome, to join the meeting ‘Crop protection: current state and prospects for human health and the environment’. The meeting addressed the consequences of using agro-chemicals, i.e. the deriving socio-economic problems and the effects of soil microbiota/microorganism, as well as alternative strategies and future perspectives. In this context, Marc Schetelig presented the ‘Genetic pathways for optimized SIT in sustainable agrigulture’. Click to read the program or watch some recorded talks of this very interesting meeting.

 

2023-10 |  Genetic Sexing Strain Research for Quarantine Species

How can we create new Genetic Sexing Strains for Bactrocera dorsalis and Bactrocera zonata, when both species have a "quarantine status" in Germany? With the help of great partners! Our laboratory collaborates again with the Insect Pest Control Laboratory (IPCL) of the Joint FAO/IAEA Centre for Nuclear Techniques in Food and Agriculture. Leveraging our history of successful collaborations with the IPCL, we are focusing on GSS development and radiation-based Sterile Insect Technique (SIT) technologies.

Further details are available in the Insect Pest Control Newsletter, Issue No. 101, on page 33.

... And if you are wondering what the International Atomic Energy Agency (IAEA) has to do with pest control, watch their animated infographic, "Using Nuclear Science to Control Pests"

 

2023-10 |  Academic delegation from CAAS-AGIS at JLU

We welcomed an academic delegation from CAAS-AGIS (Chinese Academy of Agriculture Sciences, Agricultural Genomics Institute at Shenzhen) here at JLU, to implement the SINO-German Mobility Program that are co-funded by NSFC and DFG. The scientific topic of this program is “Development of Next-Generation Control Strategies for Important Invasive Species Based on Metagenomic Analysis”. During the stay of the CAAS delegation, we have exchanged ideas for the future collaborations, such as co-publications and joint-grant opportunities. Several invasive pests that are threating the agriculture and human health in both Europe and China are identified and prioritised as potential targets for joint-research. The visiting plans for the next year within the Mobility Program were also discussed as follow-ups for this meeting.

 

2023-10 | Publication in the Int J Molecular Sciences: Improved piggyBac Transformation

Tired of low transgenesis efficiencies? Read our new publication on improved piggyBac transformation with capped transposase mRNA in pest insects and get the job done faster! Irina Häcker and colleagues show a systematic comparison of transgenesis efficiencies in Aedes mosquitoes. Suppling piggyBac transposase as capped mRNA increased the average transformation efficiency in Aedes aegypti tenfold, from less than 5% (plasmid source) to about 50%.

 

2023-08 |  iNaturalist - everyone can contribute to science

Visit the REACT Citizen Science project on iNaturalist, and help us to learn more about the dispersion of the Oriental fruit fly and the Peach fruit fly. So far, we have more than 800 observations from almost 600 observers. Many thanks!

 

2023-06 |  REACT goes on air

Cristina Borghesi talks about REACT on Radio Anaunia! Read more about our EU funded project REACT and listen to the Italian radio interview here

 

2023-05 | Publication in the Int J Molecular Sciences: Protocol for Extraction of Bacterially Produced dsRNA

Learn here how to get bacterially produced dsRNA at good yield and low costs, using an optimized acidic phenol-based protocol! Lucas Prates et al. compared the dsRNA quality and yield as well as costs of extraction of their new, optimized protocol with other protocols available in the literature.

 

2022-11 |  REACT 

       

We are very happy to announce the start of REACT! REACT stands for "Rapid elimination of invasive agricultural insect pest outbreaks by tackling them with Sterile Insect Techniques programs" and is an EU-funded project coordinated by our department. With 15 great partners from 12 countries, we are aiming to develop long-term environmentally friendly response strategies and novel sustainable tools to protect fruit and vegetable production and export capacities from two invasive insect pests of EU priority: Bactrocera dorsalis and Bactrocera zonata. Visit REACT and learn more about the project, the partners, the pests and all current activities at react-insect.eu!

 

2021-06  |  Publication in the Int J Molecular Sciences: the inducible CRISPR/Cas9 system in D. suzukii 

The paper “Highly efficient temperature inducible CRISPR-Cas9 gene targeting in Drosophila suzukii”, is now published in the International Journal of Molecular Sciences. In this effort, Ying Yan and colleagues developed the first conditional CRISPR/Cas9 system in insect species that allows temperature-controllable gene targeting. Our master student, Ms. Yukino Kobayashi, who dedicated her master thesis to the characterization of the system, is the second author of this paper. 

 

2021-06  |  Publication in Scientific Reports: the early female-killing system in D. suzukii 

The paper “A transgenic female killing system for the genetic control of Drosophila suzukii”, is now published in Scientific Reports. In this research, one of the first female-killing systems in the global pest Drosophila suzukii was developed, which can facilitate the genetic control of this species.  

 

2021-02 | Book on "Area-Wide Integrated Pest Management published by Tayler-Francis/CRC

 

This new book addresses various fundamental components of AW-IPM. It is a great collection of chapters from 184 experts from more than 31 countries and includes many technological advances in the areas of genetics, molecular biology, microbiology, resistance management, and social sciences that facilitate the planning and implementing of area-wide strategies. Our chapter on Genome Editing and its Applications for Insect Pest Control summarizes the potential of novel technologies and discusses if they are a 'curse of blessing' for the insect pest control field.

 

2021-01 | 2nd edition of the Sterile Insect Technique” book published by Tayler-Francis/CRC

 The second edition of the "bible" on the Sterile Insect Technique has been published that updated and considerably expanded the content of the first edition from 2005. A great variety of subjects of the SIT is covered, from the history to improved prospects for its future application. The major chapters discuss the principles and technical components of applying sterile insects. Other chapters deal with supportive technologies, economic, management, and environmental considerations, and the socio-economic impact of AW-IPM programs that integrate the SIT. In addition, this second edition includes six new chapters covering the latest technologies in this field. Read more on one of those chapters about Applying Modern Molecular Technologies in Support of the Sterile Insect Technique by Irina Häcker, Kostas Bourtzis and Marc F. Schetelig.

 

2021-01  |  Publication in Nature Communications: the white pupae gene.

For more than 20 years, researchers studying the field of the Sterile Insect Technique were trying to unravel the molecular basis of the white pupae marker gene. And finally – we did it, together with a large consortium and multiple approaches on several species. How? Check out our publication on Nature Communications

 

see also ProLOEWE News, Gießener Allgemeine, Press release JLU, International Atomic Energy Agency News

 

2021-01  | Publication in Gene: the β2-tubulin promoter in D. suzukii 

The paper “Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination”, is now published in Gene. This research developed one of the first sexing systems in the devastating pest Drosophila suzukii using β2-tubulin gene promoter.

 

 

 

2020-12  |  iFZ Master Award 2020 to Yukino Kobayashi, Gießen

Congratulations Yuki! 

Ms. Yukino Kobayashi won the iFZ Masters 2020 Award from the Interdisciplinary Research Center (iFZ) of the Justus-Liebig-University Giessen. Her master thesis was supervised by  Dr. Ying Yan and focused on developing genome editing tools for the invasive agricultural pest Drosophila suzukii.

 

We wish you all the best for your next steps!

 

2020-11  |  Drosophila suzukii Management” book published by Springer Nature 

 

The new book on “Drosophila suzukii Management” gathering research from leading scientists in the management of the invasive pest D. suzukii around the globe, was published by Springer Nature. We contributed the chapter “Conditional Expression Systems for Drosophila suzukii Pest Control” and our Ph.D. student Ms. Syeda A. Jaffri is the first author of this chapter. For more details about this book click here

 

 

2020-10  |  Special issue on sterile male insect strains published in BMC Genetics

Special issue “Comparing rearing efficiency and competitiveness of sterile male strains produced by genetic, transgenic or symbiont-based technologies”, edited by Kostas Bourtzis, Carlos Caceres, and Marc F. Schetelig is now published in BMC Genetics. The issue is a great collection of research papers from our collaborators and includes also four publications from our group:

1) Handler & Schetelig (BMC Genetics 21:137) evaluated the transposable element hopper from B. dorsalis;

2) Yan & Scott (BMC Genetics 21:141) developed a transgenic sexing system in Lucilia cuprina;

3) Concha et al. (BMC Genetics 21:143) generated early female lethal strains in the New World Screwworm; and

4) Yan et al. (BMC Genetics 21:146) characterized four cellularization genes from Drosophila suzukii.

 

2020-10  |  Men only! .... publication in Scientific Reports

Congratulations Rosi for the new publication in Scientific Reports! Efficient SIT programs require the release of a male-only population. Economically the best way to get that is by transforming female embryos into male adults. How’s that possible? We showed it, using CRISPR/Cas9 HDR site-specific mutagenesis on the transformer 2 gene. Check out more here!

 

2020-10  |  DAAD Scholarship for Lucas Prates!

DAAD Scholarship holder joined AG Schetelig. Since October 2020, Lucas Prates joined our research group as DAAD scholarship holder of the Programme “Research Grants – Doctoral Programmes in Germany, 2020/21”. The research project is focused on the sustainable control of the invasive disease vectors Aedes aegypti and Aedes albopictus by novel approaches. Welcome, Lucas!

 

2020-10  |  Publication in the Journal of Insect Physiology

 

Our paper “CRISPR/Cas9 mediated disruption of the white gene leads to pigmentation deficiency and copulation failure in Drosophila suzukii”, is now published in the Journal of Insect Physiology. We congratulate our student, Judith Ziemek, who did her Bachelor thesis with us, analyzing the mating behavior of the white mutants, and is the second author of this paper. 

 

 

2020-09  |  Scholarship for Ebthal Farag

Ms. Ebthal Zareef Abdalfatah Farag successfully completed her practical course in the laboratory course MP156 with the topic “Gene isolation and evaluation for CRISPR-based biocontrol strategies of the invasive pest Drosophila suzukii”. She received the “Graduation scholarship“ for international students at the JLU Gießen, which is financed by the DAAD and the State of Hesse in 2020. Congratulations Ebthal!

 

2020-08  |  ***Open for submissions until March 2022***: special issue in JIA

Our special issue “Novel Pest Control Strategies Based on Metagenomic Analysis”, Journal of Integrative Agriculture edited by Ying Yan, Max Scott, and Marc F. Schetelig, is now open for submissions. This issue welcomes original research or review articles focusing on genome research or molecular tools that could facilitate the development of genetic control strategies for agricultural pests. In addition, it also accepts articles that address the regulation of specific genetic control approaches in different countries or regions. 

 

2020-08  |  Farewell and good luck for Bashir

Our excellent technician, Bashir Hosseini, moved on to a new job. Due to his outstanding technical support, a solid and comprehensive molecular platform has been established in close collaboration with Ying in the worldwide pest Drosophila suzukii, which should greatly facilitate functional genomics and genetic control studies in this pest. We wish you, Bashir, a lot of success and happiness in the future! We’ll miss you!

  

 

2020-04  |  Publication on bicistronic protein expression in D. suzukii in IBMB

Our paper “Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides” is now published in Insect Biochemistry and Molecular Biology. This work used the picornaviral 2A peptides and different molecular tags to achieve efficient protein expression and localization in insect cells and the pest insect Drosophila suzukii. These elements will be valuable tools for genetic research in many insects. 

 

2020-04  |  First SINO-German collaboration meeting

The first joint video meeting with the Agricultural Genomics Institute of the Chinese Academy of Agricultural Sciences (CAAS) was held online under the framework of the “SINO-German Mobility Program” (DFG-NSFC, Germany-China, 2020-2022). Both sides introduced their teams, research plans and discussed the roadmap for future collaboration. We’ll continue the information exchange, project planning, and joint publication efforts, despite the hurdles of the COVID pandemic. 

The “SINO-German Mobility Program” which facilitates bilateral cooperation between top research groups from China and Germany, was awarded in Nov. 2019 to us by the DFG-NSFC (2020-2022). In this program, we will collaborate with the Agricultural Genomics Institute at the Chinese Academy of Agricultural Sciences (AGIS-CAAS, China) under the topic “Development of Next-Generation Control Strategies for Important Invasive Species Based on Metagenomic Analysis”. AGIS-CAAS is one of the leading institutes in the world that address genome research in economically important agricultural species. Combining our expertise in modern molecular techniques, sequencing tools, and bioinformatical analysis, this program aims for a better understanding of the biological invasion and provide better solutions for the agricultural landscape in both China and Germany.

 

2019-09  |  Best Talk at the 12th GGL Conference on Life Science, Gießen, Germany

 

Congratulations to our PhD Rosi for giving the best talk at the 12th Annual GGL Conference of the Life Sciences in 2019 with more than 200 presenters in total!

M. Sc. Roswitha A. Aumann

Best Talk Award: 1st Place; Title: "Seeking temperature-dependent sex reversion in Ceratitis capitata by CRISPR-Cas9 mediated gene editing of transformer-2", 04.-05. September 2019

 

2019-07  |  Best Poster at the Molecular Insect Science Conference 2019, Sitges, Spain

Great job Sarah!

M. Sc. Sarah Petermann, Dr. Sabine Otto & Prof. Marc Schetelig

 

Eighth International Symposium on Molecular Insect Science

Best Poster Award; "Genetic variability and population dynamics of Drosophila suzukii in Germany", Sitges, Spain, 7-10 July 2019

 

2019-06 |  BRUMMTIER - Gesprächsrunde zum Thema Insekten mit Prof. Marc Schetelig

 

Berlin. Farming The Uncanny Vally, 17.06.2019

 

Gesprächsrunde des BMBF geförderten Projektes Farming The Uncanny Vally über das Thema Insekten und deren Nutzung in verschiedensten Lebensbereichen. Mehr zum Projekt, den Ergebnissen, den Workshops und der Interaktion der verschiedensten Player hier.

 

  

2019-06  |  Aktionstag "Insekten (er)leben" - Es kreucht und fleucht

Gießen. Wetterauer Zeitung, 2019

"Zucker reicht. Mehr brauchen die Männchen nicht zum Leben. Ihre Weibchen sind deutlich anspruchsvoller. Es geht, na klar, um Mücken. Genauer: um die in den Tropen und Subtropen heimischen Gelbfiebermücken. Als Prof. Marc Schetelig am Samstag im Interdisziplinären Forschungszentrum (IFZ) der JLU über seine Forschungsobjekte spricht, wirken sie einen Moment lang relativ menschlich. In Wahrheit sind sie am Aktionstag »Insekten (er)leben« der Institute für Insektenbiotechnologie und Tierökologie ein Beispiel für Insekten als Gesundheitsrisiko. Ob Dengue, Zika oder andere Viren: Die Weibchen saugen nicht nur Blut - sie übertragen auch Krankheiten. Die in seinem Kasten seien aber virenfrei, verspricht Schetelig. Falls eine ausbüxt....."