Inhaltspezifische Aktionen



Häcker I, Rehling T, Schlosser H, Mayorga-Ch D, Heilig M, Yan Y, Armbruster PA, Schetelig MF (2023) Improved piggyBac transformation with capped transposase mRNA in Pest Insects. International Journal of Molecular Sciences; 24(20):15155.

Prates LHF, Merlau M, Rühl-Teichner J, Schetelig MF, Häcker I (2023) An Optimized/Scale Up-Ready Protocol for Extraction of Bacterially Produced dsRNA at Good Yield and Low Costs. International Journal of Molecular  Sciences; 25;24(11):9266.

Obermann W, Azri MFD, Konopka L, Schmidt N, Magari F, Sherman J, Silva LMR, Hermosilla C, Ludewig AH, Houhou H, Haeberlein S, Luo MY, Häcker I, Schetelig MF, Grevelding CG, Schroeder FC, Lau GSK, Taubert A, Rodriguez A, Heine A, Yeo TC, Grünweller A, Taroncher-Oldenburg G (2023) Broad anti-pathogen potential of DEAD box RNA helicase eIF4A-targeting rocaglates. Scientific Reports;13(1):9297. 

Yan Y, Hosseini B, Scheld A, Pasham S, Rehling T, Schetelig MF (2023) Effects of antibiotics on the in vitro expression of tetracycline-off constructs and the performance of Drosophila suzukii female-killing strains. Frontiers in Bioengineering and Biotechnology, 14:11:876492.

Sollazzo G, Gouvi G, Nikolouli K, Aumann RA, Djambazian H, Whitehead MA, Berube P, Chen SH, Tsiamis G, Darby AC, Ragoussis J., Schetelig MF, Bourtzis K (2023) Genomic and cytogenetic analysis of the Ceratitis capitata temperature-sensitive lethal region. G3, 13(6):jkad074

Yan Y, Aumann RA, Haecker I and Schetelig MF (2023) CRISPR-based genetic control strategies for insect pests. Journal of Integrative Agriculture. 22:651-668.

2022  (co-first= *)

Sollazzo G; Gouvi G; Nikolouli K; Cancio Martinez EI; Schetelig MF; Bourtzis K (2022) Temperature Sensitivity of Wild-Type, Mutant and Genetic Sexing Strains of Ceratitis capitata. Insects, 13:943

Schetelig MF, Lees RS, D'Amato R, Benedict MQ (2022) Inducible and Repressible Systems for Transgene ExpressionIn: Transgenic Insects - Techniques and Applications, 2nd Edition, Benedict MQ, Scott MJ (Eds.). CABI: Wallingford; ISBN : 978-1-80062-115-2, in print

Handler AM, Schetelig MF (2022) Tephritid Fruit Fly Transgenesis and Applications. In: Transgenic Insects - Techniques and Applications, 2nd Edition, Benedict MQ, Scott MJ (Eds.). CABI: Wallingford; ISBN : 978-1-80062-115-2, in print

Strobl F, Schetelig MF, Stelzer EHK (2022) In toto light-sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development. Scientific Data, 9(1):340

Xu L, Jiang HB, Tang KY, Yan Y, Schetelig MF, Wang JJ (2022) CRISPR-mediated mutagenesis of the odorant receptor co-receptor (Orco) gene disrupts olfaction-mediated behaviors in Bactrocera dorsalis. Insect Science29(5):1275-1286

Yamada H,  Dias VS,  Parker AG,  Maiga H,  Kraupa C,  Vreysen MJB,  Mamai W,  Schetelig MF,  Somda NSB,  Bouyer J (2022) Radiation dose-rate is a neglected critical parameter in dose-response of insectsScientific Reports, 12(1):6242



Dias VS, Caceres C, Parker AG, Pereira R, Demirbas-Uzel G, Abd-Alla AMM, Teets NM, Schetelig MF, Handler AM, Hahn DA (2021) Mitochondrial superoxide dismutase overexpression and low oxygen conditioning hormesis improve the performance of irradiated sterile males. Scientific Reports 

Yan Y, Kobayashi Y, Huang C, Liu B, Qian W, Wan F, Schetelig MF (2021) Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting in Drosophila suzukii. International Journal of Molecular Sciences 22(13):6724

Schetelig MF, Schwirz J and Yan Y (2021) A transgenic female killing system for the genetic control of Drosophila suzukiiScientific Report 11:12938

Petermann S, Otto S, Eichner G, Schetelig MF (2021) Spatial and temporal genetic variation of Drosophila suzukii in Germany. Journal of Pest Science 94:1291–1305

Ward CM*, Aumann RA*, Whitehead MA, Nikolouli K, Leveque G, Gouvi G, Fung E, Reiling SJ, Djambazian H, Hughes MA, Whiteford S, Caceres-Barrios C, Nguyen TNM, Choo A, Crisp P, Sim SB, Geib SM, Marec F, Häcker I, Ragoussis J, Darby AC, Bourtzis K, Baxter SW, Schetelig MF (2021) White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter. Nature Communications 12:491

Häcker I, Schetelig MF (2021) Genome editing and its applications for insect pest control: Curse or Blessing? In: Area-wide Integrated Pest Management: Development and Field Application. Hendrichs J, Pereira R, Vreysen MJB (eds.). pp 809-842. CRC Press, Boca Raton, Florida, USA.

Yan Y,  Schwirz J, Schetelig MF (2021) Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and inseminationGene 771:145366

Häcker I, Bourtzis K, Schetelig MF (2021) Applying Modern Molecular Technologies in Support of the Sterile Insect Technique. In: Sterile Insect Technique. Principles and Practice in Area-Wide Integrated Pest Management. 2nd Edition. Dyck VA, Hendrichs J, Robinson AS (eds). pp 657-702. CRC Press, Boca Raton, Florida, USA.



Häcker I, Koller R, Eichner G, Martin J, Liapi E, Rühl J, Rehling T, Schetelig MF (2020) Evaluation of hydrogen peroxide fumigation and heat treatment for standard emergency arthropod inactivation in BSL-3 insectariesFrontiers in Bioengineering and Biotechnology 8:602937.

Bourtzis K, Caceres C, Schetelig MF (2020) Joint FAO/IAEA coordinated research project on “comparing rearing efficiency and competitiveness of sterile male strains produced by genetic, transgenic or symbiont-based technologies”. BMC Genetics 21:148.

Jaffri SA, Yan Y, Scott MJ, Schetelig MF (2020) Conditional expression systems for Drosophila suzukii pest control. In: Drosophila suzukii management. Garcia FRM (eds). pp 195-215. Springer, Cham.

Jaffri SA, Yan Y, Schwirz J, Schetelig MF (2020) Functional characterization of the Drosophila suzukii pro-apoptotic genes reaperhead involution defective and grimApoptosis 25:864-874.

Aumann RA, Häcker I, Schetelig MF (2020) Female-to-male sex conversion in Ceratitis capitata by CRISPR/Cas9 HDR-induced point mutations in the sex determination gene transformer-2. Scientific Reports 10:18611.

Yan Y,  Ziemek J, Schetelig MF (2020) CRISPR/Cas9 mediated disruption of the white gene leads to pigmentation deficiency and copulation failure in Drosophila suzukii. Journal of Insect Physiology 126:104091.

Zhao Y, Schetelig MF, Handler AM (2020) Genetic breakdown of a Tet-off conditional lethality system for insect population control. Nature Communications 11, 3095.

Concha C*, Yan Y*, Arp A, Quilarque E, Sagel A, de León AP, McMillan O, Skoda S, Scott MJ (2020) An early female lethal system of the New World screwworm, Cochliomyia hominivorax, for biotechnology-enhanced sterile insect technique. BMC Genetics 21(Suppl 2):143.

Yan Y, Scott MJ (2020) Building a transgenic sexing strain for genetic control of the Australian sheep blow fly Lucilia cuprina using two lethal effectors. BMC Genetics 21(Suppl 2):141.

Yan Y, Williamson ME, Scott MJ (2020) Using moderate transgene expression to improve the genetic sexing system of the Australian Sheep Blow fly Lucilia cuprina. Insects 11(11):797.

Liu GQ, Lv, Z, Wu Q, Zhou ZS, Zhang, GF, Wan, FH, Yan Y (2020) The homology gene of Bdcp-1 is essential for female fertility in Bactrocera dorsalis. Pest Management Science, 76: 4104–4111.

Yamada H, Maiga H, Bimbile-Somda NS, Carvalho DO, Mamai W, Kraupa C, Parker AG, Abrahim A, Weltin G, Wallner T, Schetelig MF, Caceres C, Bouyer J (2020) The role of oxygen depletion and subsequent radioprotective effects during irradiation of mosquito pupae in water. Parasites & Vectors 13, 198.

Handler AM, Schetelig MF (2020) The hAT-family transposable element, hopper, from Bactrocera dorsalis is a functional vector for insect germline transformation. BMC Genetics 21:137.

Yan Y, Jaffri SA, Schwirz J, Stein C, Schetelig MF (2020) Identification and characterization of four Drosophila suzukii cellularization genes and their promoters. BMC Genetics 21:146.

Schwirz J*, Yan Y*, Franta Z, Schetelig MF (2020) Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptidesInsect Biochemistry and Molecular Biology 119: 103324.



Giese B, Frieß JL, Barton NH, Messer PW, Débarre F, Schetelig MF, Windbichler N, Meimberg H, Boëte C (2019) Gene Drives: Dynamics and Regulatory Matters. BioEssays 2019, 1900151

Schwirz J*, Yan Y*, Franta Z, Schetelig MF (2019) Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides. BioRxiv:

Teets N, Dias VS, Pierce B, Schetelig MF, Handler AM, Hahn D (2019) Overexpression of an antioxidant enzyme improves male mating performance after stress in a lek-mating fruit fly. Proceedings of the Royal Society B 286: 20190531

Schetelig MF (2019) Genomic engineering of insects for sustainable crop protection. Atti Accademia Nazionale Italiana di Entomologia Anno LXVI, 2018: pp. 111-114 

Spielmeyer A, Schetelig MF, Etang J (2019) High-throughput analysis of insecticides on malaria vectors using liquid chromatography tandem mass spectrometry. PLOS One 14(2):e0211064

Schetelig MF, Yan Y, Zhao Y, Handler AM (2019) Genomic targeting by recombinase-mediated cassette exchange in the spotted wing drosophila, Drosophila suzukiiInsect Molecular Biology 28(2):187-195



Häcker I, Schetelig MF (2018) Molecular tools to create new strains for mosquito sexing and vector control. Parasites & Vectors 11 (Suppl 2): 645.

Schetelig MF, Schwirz J, Yan Y. Multiprotein expression systems for pest control (2019) Patent: EP18208613

Aumann RA, Schetelig MF, Häcker I (2018) Highly efficient genome editing by homology-directed repair using Cas9 protein in Ceratitis capitataInsect Biochemistry and Molecular Biology 101:85-93.

Ramírez-Santos E, Rendón P, Bourtzis K, Schetelig MF, Cáceres C, Targovska A, Rehling T, Guillén K, Ruiz-Montoya L, Toledo J, Liedo P (2018) Evaluation of horizontal gene transfer risk between the Mediterranean fruit fly Ceratitis capitata (Tephritidae) and its parasitoid Fopius ceratitivorus (Braconidae). PLoS One 13(12): e0207999.

Schetelig MF, Lee KZ, Otto S, Talmann L, Stökl J, Degenkolb T, Vilcinskas A, Halitschke R (2018) Environmentally sustainable pest control options for Drosophila suzukiiJournal of Applied Entomology 142(1-2): 3-17.



Häcker I, Aumann R, Schetelig MF (2017) Vector control strategies for mosquitoes. Zeitschrift für Naturforschung C, 72(9-10), e11.

Häcker I, Harrell II RA, Eichner G, Pilitt KL, O’Brochta DA, Handler AM, Schetelig MF (2017) Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti. Scientific Reports 7:43883.

Kalajdzic P, Schetelig MF (2017) CRISPR/Cas-mediated gene editing using purified protein in Drosophila suzukii (Matsumura). Entomologia Experimentalis et Applicata 164(3): 350-362. 

Papanicolaou A, Schetelig MF, et al. (2017) Erratum to: The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species. Genome Biology 18(11).


Genç H, Schetelig MF, Nirmala X, Handler AM (2016) Germline transformation of the olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), with a piggyBac transposon vector. Turkish Journal of Biology 40: 845-855. 

Papanicolaou A, Schetelig MF, Arensburger P, Atkinson PW, Benoit JB, Bourtzis K, Castañera P, Cavanaugh JP, Chao H, Childers C, Curril I, Dinh H, Doddapaneni H, Dolan A, Dugan S, Friedrich M, Gasperi G, Geib S, Georgakilas G, Gibbs RA, Giers SD, Gomulski LM, González-Guzmán M, Guillem-Amat A, Han Y, Hatzigeorgiou AG, Hernández-Crespo P, Hughes DS, Jones JW, Karagkouni D, Koskinioti P, Lee SL, Malacrida AR, Manni M, Mathiopoulos K, Meccariello A, Murali SC, Murphy TD, Muzny DM, Oberhofer G, Ortego F, Paraskevopoulou MD, Poelchau M, Qu J, Reczko M, Robertson HM, Rosendale AJ, Rosselot AE, Saccone G, Salvemini M, Savini G, Schreiner P, Scolari F, Siciliano P, Sim SB, Tsiamis G, Ureña E, Vlachos IS, Werren JH, Wimmer EA, Worley KC, Zacharopoulou A, Richards S, Handler AM (2016) The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species. Genome Biology 17(1): 1-17. 

Schetelig MF, Targovska A, Meza JS, Bourtzis K, Handler AM (2016) Tetracycline-suppressible female lethality and sterility in the Mexican fruit fly, Anastrepha ludens. Insect Molecular Biology 25(4): 500-508.

Schwirz J, Fischbach M, Vilcinskas A, Fischer R, Schetelig MF (2016) Monitoring data and future control possibilities for Drosophila suzukii in Germany. In: Proceedings of the 9th Int Symposium on Fruit Flies of Economic Importance 310-322.


Nirmala X, Schetelig MF, Zimowska GJ, Zhou L, Handler AM (2015) Pro-apoptotic gene regulation and its activation by gamma-irradiation in the Caribbean fruit fly, Anastrepha suspensa. Apoptosis 20(1): 1–9.


Gilles J, Schetelig MF, Scolari F, Marec F, Capurro ML, Franz G, Bourtzis K (2014) Towards mosquito sterile insect technique programmes: Exploring genetic, molecular, mechanical and behavioural methods of sex separation in mosquitoes. Acta Tropica 132S, S178–S187. 

Handler AM, Schetelig MF (2014) Tephritid Fruit Fly Transgenesis and Applications. In: Transgenic Insects - Techniques and Applications, Benedict MQ (Ed.), 117–137. CABI: Wallingford  

Hokanson KE, Dawson WO, Handler AM, Schetelig MF, St Leger RJ (2014) Not all GMOs are crop plants: non-plant GMO applications in agriculture. Transgenic Research 23(6): 1057–1068. 

Meza JS, Díaz-Fleischer F, Sánchez-Velásquez LR, Zepeda-Cisneros CS, Handler AM, Schetelig MF (2014) Fitness cost implications of phiC31-mediated site-specific integrations in target-site strains of the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae). PLoS One 9(10): e109690, 1–10. 

Meza JS, Schetelig MF, Zepeda-Cisneros CS, Handler AM (2014) Male-specific Y-linked transgene markers to enhance biologically-based control of the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae). BMC Genetics 15 Suppl2: S4, 1-7. 

Scolari F, Yuval B, Gomulski LM, Schetelig MF, Gabrieli P, Bassetti F, Wimmer EA, Malacrida AR, Gasperi G (2014) Polyandry in the medfly – shifts in paternity mediated by sperm stratification and mixing. BMC Genetics 15 Suppl 2: S10, 1–8. 


Nirmala X, Schetelig MF, Yu F, Handler AM (2013) An EST database of the Caribbean fruit fly, Anastrepha suspensa (Diptera: Tephritidae). Gene 517(2): 212-217. 

Ogaugwu CE, Schetelig MF, Wimmer EA (2013) Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethality. Insect Biochemistry and Molecular Biology 43(1): 1-8.

Schetelig MF, Handler AM (2013) Germline transformation of the spotted wing drosophilid, Drosophila suzukii, with a piggyBac transposon vector. Genetica 141(4-6): 189−193. 

Schetelig MF, Handler AM (2013) Y-linked markers for improved population control of the tephritid fruit fly pest, Anastrepha suspensa. Advances in Biochemical Engineering/Biotechnology 136: 123-133. 

Schetelig MF, Handler AM (2013) A functional comparison of the 3xP3 Promoter by recombinase-mediated cassette exchange in Drosophila and a Tephritid Fly, Anastrepha suspensa. G3: Genes, Genomes, Genetics 3(4): 687–693. 


Schetelig MF, Handler AM (2012) Strategy for enhanced transgenic strain development for embryonic conditional lethality in Anastrepha suspensa. PNAS 24: 9348-9353.

Schetelig MF, Handler AM (2012) A transgenic embryonic sexing system for Anastrepha suspensa (Diptera: Tephritidae). Insect Biochemistry and Molecular Biology 42: 790-795.

Schetelig MF, Milano A, Saccone G, Handler AM (2012) Male only progeny in Anastrepha suspensa by RNAi-induced sex reversion of chromosomal females. Insect Biochemistry and Molecular Biology 42: 51-57.


Schetelig MF, Nirmala X, Handler AM (2011) Pro-apoptotic cell death genes, hid and reaper, from the tephritid pest species, Anastrepha suspensa. Apoptosis 16: 759-768.

Schetelig MF, Wimmer EA (2011) Insect Transgenesis and the Sterile Insect Technique. In: Insect Biotechnology, Vilcinskas A (Eds.), pp 169-194.


Schetelig MF, Götschel F, Viktorinová I, Handler AM, Wimmer EA (2010) Recombination technologies for enhanced transgene stability in bioengineered insects for the Sterile Insect Technique. Genetica 139: 71-78.

Schetelig MF, Ogaugwu CE, Wimmer EA (2010) Development of a transgenic sexing system based on female-specific embryonic lethality in Ceratitis capitata (Diptera: Tephritidae). In: Proceedings of the 8th International Symposium on Fruit Flies of Economic Importance, Valencia, Spain, 2010. pp 106-118.


Schetelig MF, Caceres C, Zacharopoulou A, Franz G, Wimmer EA (2009) Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata (Diptera: Tephritidae). BMC Biology 7(4): 1-13.

Schetelig MF, Scolari F, Handler AM, Kittelmann S, Gasperi G, Wimmer EA (2009) Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata. PNAS 106(43): 18171-18176.


Schetelig MF, Schmid BGM, Zimowska G, Wimmer EA (2008) Plasticity in mRNA expression and localization of orthodenticle within higher Diptera Plasticity in mRNA expression and localization of orthodenticle within higher Diptera. Evol Dev. 10(6): 700-704.

Schetelig MF, Scolari F, Handler AM, Gasperi G, Wimmer EA (2008) New genetic tools for improving SIT in Ceratitis capitata: embryonic lethality and sperm marking. In:  Proceedings fo the 7th International Symposium on Fruit Flies of Economic Importance. Sugayama RL, Zucchi RA, Ovruski SM, Sivinski J (Eds.) Salvador, Bahia, Brazil 2006. pp 299-305.

Scolari F, Schetelig MF, Bertin S, Malacrida AR, Gasperi G, Wimmer EA (2008) Fluorescent Sperm Marking to Improve the Fight against the Pest Insect Ceratitis capitata (Wiedemann; Diptera: Tephritidae). New Biotechnology 25(1): 76-84.

Scolari F, Schetelig MF, Gabrieli P, Siciliano P, Gomulski LM, Karam N, Wimmer EA, Malacrida AR, Gasperi G (2008) Insect transgenesis applied to tephritid pest control. Journal of Applied Entomology 132(9-10): 820-831.


Schetelig MF, Horn C, Handler AM, Wimmer EA (2007) Development of an embryonic lethality system for transgenic SIT in the fruit pest Ceratitis capitata. In: Area-Wide Control of Insect Pests: From Research to Field Implementation. Vreysen, MJB, Robinson AS, Hendrichs, J (Eds.), pp 85-93.