Inhaltspezifische Aktionen

Epigenomics

Scientific Manager

Prof. Dr. Till Acker
FB11, Institute of Neuropathology

Deputy Manager

Prof. Dr. Attila Németh
FB11, Institute of Neuropathology, Molecular Neurooncology and Neuropathology

Technical Manager

Carmen Selignow
FB11, Institute of Neuropathology, Molecular Neurooncology and Neuropathology

Contact Information

Neuropathologie.Gi@uk-gm.de
Attila.Nemeth@patho.med.uni-giessen.de

Description

The Epigenomics Technology Module provides a platform for microarray-based genome-wide DNA methylation and hydroxymethylation analyses.

The Epigenomics TM is located at the Science Unit for Basic and Clinical Medicine (ForMED), Aulweg 128, 35392 Giessen in the Molecular Neurooncology and Neuropathology laboratory of the Institute of Neuropathology. The TM runs the Infinium MethylationEPIC (Illumina Inc.) technology.

The Epigenomics TM is open to collaborations with all groups of the JLU and external institutions. It is directed by Prof. Till Acker. For detailed information and guidelines on sample submission and costs, you can contact the TM via email.

Instrumentation/Software

The Infinium MethylationEPIC v2.0 BeadChip Kit is a genome-wide methylation screening tool that targets over 935,000 CpG sites in the most biologically significant regions of the human methylome. Microarray scanning is performed with the iScan System.

Illumina Chip and iScan System (Courtesy of Illumina Inc.)

Primary data analysis and quality control of hybridization and scanning is supported by the Methylation Module of the GenomeStudio software.

• DNA quality control (For optimal results we need >500 ng human genomic DNA with OD260/280=1.8-2.0).

• Microarray hybridization and primary data analysis.

Optional:

  • Bisulfite conversion of submitted DNA.
  • Analysis of FFPE samples (after additional fragment size QC).
  • Mouse DNA methylome and hydroxymethylome analyses using the Infinium Mouse Methylation BeadChip (>285,000 markers across the methylome for high-resolution epigenetic analyses of diverse murine strains) are possible upon request.
  • Oxidative bisulfite conversion and its QC have to be performed by the submitter.