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Instruments

 

 

    

 

Our setup is a dual 2-photon system, consisting of an inverted and an upright setup. Both systems can be operated individually as confocal microscopes while they can share a multi-photon laser from SpectraPhysics.

 

 

 

Slide Scanner

The VS200 Olympus slide scanner offers a capacity of up to 180 slides (1x3 inches). The imaging techniques offered are the following:

Brigthfield Fluorescence
Brightfield transmitted  DAPI
Darkfied FITC
Circular polarisation Cy3
Linear polarisation Cy5
  Cy7 

Objective lenses

The scanner is equipped with the following objectives:

  • 2x (for slides' label scans)
  • 4x0.13NA
  • 10x0.40NA
  • 20x0.80NA
  • 40x0.95NA
  • 100xOil1.45NA

 

 Inverted microscope

The inverted system has an Acousto-Optical Beam Splitter (AOBS), with a z-galvo stage and a resonant scanner, offering the following:

Confocal Laser Scanning Microscopy

    • Lasers:
      • 405nm
      • Argon laser (458nm, 476nm, 488nm, 496nm and 514nm)
      • White Light Laser (470nm-670nm)
    • Detectors:
      • 1 Transmitted Light PMT
      • 1 PMT
      • 3 Hybrid Detectors

Multi-photon Laser Scanning Microscopy

    • Laser:
      • SpectraPhysics Insight X3 with a 1045nm fixed line and a tuned line from 680nm up to 1300nm.
    • Detectors:
      • 4-Tune Mutli-photon module with 2 PMTs and 2HyDs

Objectives

        • 1.6x air, 2.5x air, 5x air, 10x air, 20x air
        • 25xWater, 40xWater, 63xWater (with motor correction collar)
        • 63xOil
        • 5x water diving objective for light sheet
        • 10x multi-immersion diving objective for light sheet
        • 5mm water, 7.8mm water/Glyc/BABB TwinFlect mirrors 

Software Modules

        • Lightning for online computational clearing
        • FRET and FRAP module
        • LiveData Module
        • Spectral Dye Separation

Live Imaging Setup

The inverted microscope is also equipped with a temperature controller as well as a gas mixer for CO2, providing the option for live cell imaging.

 

 

 

Upright Microscope

The upright microscope is equipped with a Scientifica XY stage and a resonant scanner for fast imaging and can offer the following:

Confocal Laser Scanning Microscopy

    • Lasers:
      • 405nm
      • 488nm
      • 552nm
      • 638nm
    • Detectors:
      • 3 PMT
      • 1 Hybrid Detector

Multi-photon Laser Scanning Microscopy

    • Laser:
      • SpectraPhysics Insight X3 with a 1045nm fixed line and a tuned line from 680nm up to 1300nm.
    • Detectors:
      • 4-Tune Mutli-photon module with 2 PMTs and 2HyDs

Objectives

        • 25x/1.00NA water diving with motor correction collar
        • 25x/1.00NA multi-immersion diving with motor correction collar

Software Modules

        • Lightning for online computational clearing
        • Spectral Dye Separation

 

 

 

 

 

 

 

 

 

 

 

AVI

 

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