WiSe 2017

D ate

T im e

T opics

08NOV2017

18:15

Stoichiometry

15NOV2017

18:15

PCR: semi-quantitative versus real-time: utility for genotyping, quantitative detection of gene expression and identification of single nucleotide polymorphisms

( L eili JAFARI)

Resolving DNA and RNA by agarose gel electrophoresis:

detection by Southern and northern blot

( Julia SCHÄFFER)

22NOV2017

18:15

Gene cloning for protein expression, including the utility and application of site-directed mutagenesis and epitope tagging

( A kis PANAGIOTIDIS)

Transcriptomic analysis: contrasting RNA-Seq with microarray approaches, including the basic principles of both techniques

( Siavash MANSOURI)

06DEC2018

Practical applications of theory

17JAN2018

18:15

Principles of preparative chromatography and protein precipitation for the fractionation of complex mixtures

( L udmila SPERLING)

Protein resolution by electrophoresis, including sample preparation and protein quantification methods

( Fabienne KNAPP)

24JAN2018

18:15

Post-translation modifications of proteins (including detection)

( D avid KLAUS)

Assessment of cell proliferation and cell death

( Wafaa MAHMOUD )

31JAN2018

Practical applications of theory

07FEB2018

18:15

Antibodies: generation and labelling of antibodies, and application in immunoblotting and immunoprecipitation

( Sophie FEDDERSEN)

Immunohistochemistry and in situ hybridization in the lung: focus on lung cell-type markers

( B eate SCHLÜTER )

14FEB2018

18:15

Transcriptome interference: Plasmids and siRNA delivery to eukaryotic cells by transfection

( N eslihan SEVINC)

Protein-protein interactions: Utility of GST pulldown and co- immunoprecipitation, including independent validation

( A nika NOLTE )

21FEB2018

18:15

Practical applications of theory

28FEB2018

18:15

Exam revision