WiSe 2022

Date

Time

Theme

Venue ^§

Examinable?

19.10.2022

PCR: semi-quantitative versus real-time: Utility for genotyping, quantitative detection of gene expression and identification of single nucleotide polymorphisms

(Martha Candoni)

Resolving DNA and RNA by agarose
gel electrophoresis: Detection by
Southern and northern blot
(Anis Cilic)

Gene cloning for protein expression,
including the utility and application of
site-directed mutagenesis and epitope
tagging
(Yash Dasadia)

Transcriptomic analysis:
contrasting RNA-Seq with
microarray approaches, including the
basic principles of both techniques
(Alejandro Egea Zorrilla)

Principles of preparative
chromatography and protein
precipitation for the fractionation of
complex mixtures
(Bhavika Kataria)

Protein resolution by electrophoresis,
including sample preparation and
protein quantification methods
(Vanessa Kleinhenn)

Antibodies: generation and labelling
of antibodies, and application in
immunoblotting and
immunoprecipitation
(Dongzhu Li)

Enzyme-linked immunosorbent assay
(ELISA)
(Muchen Li)

No tutorial

Protein-protein interactions: Utility of GST pulldown and
coimmunoprecipitation, including independent validation
(Mahtab Shahriari Felordi)

Post-translation modifications of
proteins (including detection)
(Leila Sotoodeh Atefi)

Practical applications of theory

Cell culture (primary cells versus cell
lines, cell immortalization, co-culture
models, air-liquid interface cultures,
organoids)
(Stephanie Stephan)

Flow cytometry and fluorescenceactivated
cell sorting
(Ornella Yamdjeu Tchokondu)

Assessment of cell proliferation,
migration and cell death
(Mahsa Zabihi)

Transcriptome interference: Plasmids
and siRNA delivery to eukaryotic
cells by transfection
(Martha Candoni)

Immunohistochemistry, immunofluorescence, in situ hybridization and FISH
in the lung: Focus on lung cell-type markers
(Anis Cilic)