WiSe 2024

MBML Graduate Program First-Year Tutorial Schedule: Winter Semester 2024/2025

Date	Day*			Time			Theme					Venue§			Examinable?
16.10.2024	We			18:15			*Stoichiometry*					CIGL ¹			Yes
23.10.2024	We			18:15			PCR: semi-quantitative versus real-time: Utility for genotyping, quantitative detection of gene expression and identification of single nucleotide polymorphisms (Hanieh Anari Beigdiloo)					CIGL ¹			Yes
30.10.2024	We			18:15			Gene cloning for protein expression, including the utility and application of site-directed mutagenesis and epitope tagging (Nadezhda Nikulina)		Transcriptomic analysis: contrasting RNA-Seq with microarray approaches, including the basic principles of both techniques (Derrick Opoku)			CIGL ¹			Yes
06.11.2024	We			18:15			Principles of preparative chromatography and protein precipitation for the fractionation of complex mixtures (Ashutosh Tripathy)		Protein resolution by electrophoresis, including sample preparation and protein quantification methods (Laura-Marie Twardowski)			CIGL ¹			Yes
13.11.2024	We			18:15			Protein-protein interactions: Utility of GST pulldown and coimmunoprecipitation, including independent validation (Rudy)		Post-translational modifications of proteins (including detection) (Fatemeh Zarei)			CIGL ¹			Yes
20.11.2024	We			17:00			Resolving DNA and RNA by agarose gel electrophoresis: Detection by Southern and northern blot (Rudy)		Practical application of theory			CIGL ¹			Yes
27.11.2024	We			17:00			Practical application of theory					CIGL ¹			Yes
04.12.2024	We			18:15			Antibodies: generation and labelling of antibodies, and application in immunoblotting and immunoprecipitation (Hanieh Anari Beigdiloo)		Enzyme-linked immunosorbent assay (ELISA) (Fatemeh Zarei)			CIGL ¹			Yes
11.12.2024	We			-			No tutorial					-			-
15.01.2025	We			-			No tutorial					-			-
22.01.2025	We			18:15			Cell culture: Primary cells versus cell lines, cell immortalization, co-culture models, air-liquid interface cultures and organoids (Nadezhda Nikulina)		Flow cytometry and fluorescenceactivated cell sorting (Derrick Opoku)			CIGL ¹			Yes
29.01.2025	We			18:15			Assessment of cell proliferation, migration and cell death (Ashutosh Tripathy)		Transcriptome interference: Plasmids and siRNA delivery to eukaryotic cells by transfection (Laura-Marie Twardowski)			CIGL ¹			Yes
05.02.2025	We			18:15			Immunohistochemistry, immunofluorescence, in situ hybridization and FISH in the lung: Focus on lung cell-type markers (Zahra Moazezi Therankhah)					CIGL ¹			Yes
12.02.2025	We			18:15			Practical application of theory					CIGL ¹			Yes
19.02.2025	We			-			No tutorial					-			-
26.02.2025	We			17:00			Exam Revision					CIGL ¹			-
12.03.2025	We			09:00			Examination					Anatomie ²			-
19.03.2025	We			09:00			Re-examination					Anatomie ²			-

*Day key:	We, Wednesday
^§ Venue key:	¹ Seminar Room, CIGL Building (ground floor), Aulweg 132 ² Kursraum, Institute for Anatomy and Cell Biology, Aulweg 121