Research

Eukaryotic genomes are organized into distinct chromatin domains that are transcriptionally active (euchromatin) or repressed (heterochromatin). Many of the structural components and enzymes contributing to heterochromatin formation have been identified. Once established, heterochromatin has the propensity to spread along the chromosome and is stably maintained through mitotic and meiotic cell divisions (epigenetic inheritance). However, we still have a poor knowledge of the spatiotemporal control of these processes: What favors the formation of heterochromatin? Which factors contribute to these processes? What are the underlying mechanisms? What limits heterochromatin spreading beyond natural boundaries? How are these different pathways coordinated? How are they dynamically regulated?

To address these questions, our lab applies several strategies. Using functional genome-wide screens, we seek to identify novel factors that modulate the heterochromatic state. Using functional genomics, we examine how these factors cooperate by dissecting regulatory pathways and networks. Using live-cell imaging, molecular biology and biochemistry methods, we investigate the mechanisms of heterochromatin regulation by these factors.