Flow Cytometry (FACS)
ManagementScientific Management Technical Management |
Contact InformationMartin Speckmann Consulting Hours |
Bild: Alexander Hohberger (2025)
Description
The JLU Flow Core provides state-of-the-art equipment, hands-on support and tailored training courses in the field of Flow Cytometry for all JLU research groups as well as for interested external academic and industrial scientists. Covered technologies and services range from conventional to full spectrum multi-parameter cell analysis, cell sorting and data analysis. As a full service facility meeting researchers' specific needs is our priority by guiding through the complete workflow of flow cytometry experiments, starting from planning and execution to evaluation and illustration.
Instrumentation/Software
Flow Cytometer
| Flow Cytometer | Cytometer Type | DFG Instrumentation Class | Detection Technology | Colors | Construction Year | Manufacturer |
|---|---|---|---|---|---|---|
| BD FACSCanto II | Cell Analyzer | Class I | Conventional (Bandpass Filter) | 8 | 2007 | BD Biosciences |
| Sony SP6800 Spectral Analyzer | Cell Analyzer | Class I | Full Spectrum | 16 | 2017 | Sony Biotechnology |
| BD FACSMelody | Cell Sorter | Class III | Conventional (Bandpass Filter) | 8 | 2021 | BD Biosciences |
| BD FACSymphony S6 | Cell Sorter | Class III | Conventional (Bandpass Filter) | 30 | 2022 | BD Biosciences |
Software
| Software | Version | Manufacturer | Applications |
|---|---|---|---|
| FACSDiva | v9 | BD Biosciences | Basic Analysis, Worksheet Preparation |
| FlowJo | v10 | BD Biosciences | Advanced Analysis, Stats, Illustration |
| SP6800 | – | Sony Biotechnology | Spectral Flow Cytometry Analysis |
| SeqGeq | v1 | BD Biosciences | scRNAseq & Flow Data Analysis |
| GraphPad Prism | v10 | GraphPad Software | Statistics, Figure Illustration |
Services
Administration
- Support and cooperation for large equipment applications
Self-Service
- Training courses (instruments: BD FACSCanto II, Sony SP6800, BD FACSMelody; software: FlowJo)
- Provision of software for on-site use or rent
- Support for: experiment design, sample prep, panel establishment, instrument operation, data acquisition/sorting, evaluation
Service
- Antibody validation
- Cell analysis and sorting (single/bulk)
- Data analysis: conventional, dimensionality reduction, incl. biostatistics
For further information, please contact the scientific management of the Core Facility.